RT Journal Article SR Electronic(1) A1 Park, Seong Chan A1 Kim, Mi Sun A1 Baik, Keun Sik A1 Kim, Eun Mi A1 Rhee, Moon Soo A1 Seong, Chi NamYR 2008 T1 Chryseobacterium aquifrigidense sp. nov., isolated from a water-cooling system JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 3 SP 607 OP 611 DO https://doi.org/10.1099/ijs.0.65475-0 PB Microbiology Society, SN 1466-5034, AB A non-motile, yellow-pigmented bacterium, designated strain CW9T, was isolated from a water-cooling system in Gwangyang, Republic of Korea. The cells were Gram-negative, catalase- and oxidase-positive, short rods. The major fatty acids were iso-C15 : 0 (45.5 %), iso-C17 : 1 ω9c (14.4 %), iso-C17 : 0 3-OH (13.0 %) and summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1 ω7c; 8.8 %). The DNA G+C content was 35.6 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain CW9T formed a lineage within the genus Chryseobacterium and was closely related to Chryseobacterium gleum ATCC 35910T (98.4 % sequence similarity) and Chryseobacterium indologenes ATCC 29897T (97.8 % sequence similarity). Phenotypic characteristics and DNA–DNA relatedness data served to distinguish strain CW9T from these two species. On the basis of the evidence presented in this study, strain CW9T represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium aquifrigidense sp. nov. is proposed. The type strain is CW9T (=KCTC 12894T=JCM 14756T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65475-0