1887

Abstract

Five bacterial isolates were recovered from dermatitis or organ lesions of five agamid lizards. Three strains were recovered from species with dermatitis or septicaemia. All five isolates were Gram-positive small rods that could not be identified using conventional phenotypic systems. They grew on sheep blood agar as small haemolytic colonies after 24 h of incubation at temperatures between 25 and 42 °C under aerobic, microaerophilic or anaerobic conditions. They were catalase-positive and non-motile. Comparative analysis of 16S rRNA gene sequences revealed that the strains represent a new taxon within the class . Their nearest phylogenetic neighbours were determined as LMG 19847 (95.9 % 16S rRNA gene sequence similarity) and NCIMB 13131 (95.3 % similarity). The DNA G+C content of one of the novel isolates, strain IMP2, was 61 mol%. On the basis of morphological, chemotaxonomic and phylogenetic differences from other species of coryneform bacteria, it is proposed that this novel taxon be classified as gen. nov., sp. nov. The type strain is IMP2 (=LMG 24257=CCUG 55056).

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2008-09-01
2024-03-28
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References

  1. Baele, M., Baele, P., Vaneechoutte, M., Storms, V., Butaye, P., Devriese, L., Verschraegen, G., Gillis, M. & Haesebrouck, F.(2000). Application of tRNA intergenic spacer PCR for identification of Enterococcus species. J Clin Microbiol 38, 4201–4207. [Google Scholar]
  2. Baele, M., Chiers, K., Devriese, L. A., Smith, H. E., Wisselink, H. J., Vaneechoutte, M. & Haesebrouck, F.(2001). The gram-positive tonsillar and nasal flora of piglets before and after weaning. J Appl Microbiol 91, 997–1003.[CrossRef] [Google Scholar]
  3. Buczolits, S., Schumann, P., Weidler, G., Radax, C. & Busse, H.-J.(2003).Brachybacterium muris sp. nov., isolated from the liver of a laboratory mouse strain. Int J Syst Evol Microbiol 53, 1955–1960.[CrossRef] [Google Scholar]
  4. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  5. Lanyi, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  6. MacKenzie, S. L.(1987). Gas chromatographic analysis of amino acids as the N-heptafluorobutyryl isobutyl esters. J Assoc Off Anal Chem 70, 151–160. [Google Scholar]
  7. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  8. Pasmans, F., Blahak, S., Martel, A. & Pantchev, N.(2008). Introducing reptiles into a captive collection: the role of the veterinarian. Vet J 175, 53–68.[CrossRef] [Google Scholar]
  9. Pitcher, D. G., Saunders, N. A. & Owen, R. J.(1989). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef] [Google Scholar]
  10. Rhuland, L. E., Work, E., Denman, R. F. & Hoare, D. S.(1955). The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77, 4844–4846.[CrossRef] [Google Scholar]
  11. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  12. Schleifer, K. H.(1985). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156. [Google Scholar]
  13. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  14. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  15. Vandamme, P., Vancanneyt, M., Pot, B., Mels, L., Hoste, B., Dewettinck, D., Vlaes, L., Van Den Borre, C., Higgins, R. & other authors(1992). Polyphasic taxonomic study of the emended genus Arcobacter with Arcobacter butzleri comb. nov. and Arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. Int J Syst Bacteriol 42, 344–356.[CrossRef] [Google Scholar]
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