RT Journal Article SR Electronic(1) A1 Bang, Didi A1 Herlin, Troels A1 Stegger, Marc A1 Andersen, Aase Bengaard A1 Torkko, Pirjo A1 Tortoli, Enrico A1 Thomsen, Vibeke OstergaardYR 2008 T1 Mycobacterium arosiense sp. nov., a slowly growing, scotochromogenic species causing osteomyelitis in an immunocompromised child JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 10 SP 2398 OP 2402 DO https://doi.org/10.1099/ijs.0.65503-0 PB Microbiology Society, SN 1466-5034, AB A yellow-pigmented, scotochromogenic, slowly growing mycobacterial strain, designated T1921T, was isolated from the disseminated osteomyelitic lesions of a 7-year-old child with an underlying partial gamma interferon receptor alpha-1 deficiency. Hybridization by the line probe assay indicated the presence of a Mycobacterium species. Sequencing of the 16S rRNA gene, the internally transcribed spacer (ITS) region and the hsp65 and rpoB genes revealed that strain T1921T could be differentiated from all recognized species of the genus Mycobacterium. Phylogenetic analysis based on the 16S rRNA gene indicated that strain T1921T was related most closely to Mycobacterium intracellulare, whereas analysis based on the ITS and hsp65 and rpoB genes indicated that it was most closely related to Mycobacterium avium. Phenotypic tests were not able to differentiate strain T1921T from similar slowly growing mycobacteria. Strain T1921T is considered to represent a novel species of the genus Mycobacterium, for which the name Mycobacterium arosiense sp. nov. is proposed. The type strain is T1921T (=DSM 45069T =ATCC BAA-1401T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65503-0