1887

Abstract

A moderately alkaliphilic and halophilic bacterium was isolated from sediment of Xiarinaoer soda lake located in the Inner Mongolia municipality. This bacterium, designated strain H-5, was a facultative anaerobe, Gram-positive, rod-shaped and non-motile. Strain H-5 grew in complex medium with 0.5–30 % (w/v) NaCl and at pH 7.5–13. The cell wall peptidoglycan contained -diaminopimelic acid. The major isoprenoid quinones found in this strain were MK-9H and MK-9H, and the major cellular fatty acids were C and anteiso-C. The DNA G+C content of strain H-5 was 38.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain H-5 was located in the genus . The 16S rRNA gene sequence similarities between strain H-5 and the type strains of the two recognized species of the genus were 98.6 and 98.0 %. The DNA–DNA relatedness values between strain H-5 and the two type strains were 19 and 5 %. Based on the phenotypic and chemotaxonomic data, the phylogenetic analysis and genomic distinctiveness, strain H-5 is considered to represent a novel species of the genus , for which the name is proposed. The type strain is H-5 (=CGMCC AS 1.6843=NBRC 103919).

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2008-09-01
2024-04-16
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References

  1. Altschul, S. F., Madden, T., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef] [Google Scholar]
  2. Brosius, J., Palmer, J. L., Kennedy, J. P. & Noller, H. F.(1978). Complete nucleotide sequence of a 16S ribosomal RNA gene from Escherichia coli. Proc Natl Acad Sci U S A 75, 4801–4805.[CrossRef] [Google Scholar]
  3. Collins, M. D.(1985). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  4. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  5. Dong, X.-Z. & Cai, M.-Y.(2001).Determinative Manual for Routine Bacteriology. Beijing: Scientific Press.
  6. Duckworth, A. W., Grant, W. D., Jones, B. E. & van Steenbergen, R.(1996). Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiol Ecol 19, 181–191.[CrossRef] [Google Scholar]
  7. Gregersen, T.(1978). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef] [Google Scholar]
  8. Güssow, D. & Clackson, T.(1989). Direct clone characterization from plaques and colonies by the polymerase chain reaction. Nucleic Acids Res 17, 4000[CrossRef] [Google Scholar]
  9. Hasegawa, T., Takizawa, M. & Tanida, S.(1983). A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  10. Horikoshi, K. & Grant, W. D. (editors)(1998).Extremophiles, vol. 6, pp. 155–168. Edited by Ralph Mitchell. Division of Applied Sciences, Cambridge, USA: Harvard University.
  11. Ishikawa, M., Nakajima, K., Itamiya, Y., Furukawa, S., Yamamoto, Y. & Yamasato, K.(2003).Marinilactibacillus psychrotolerans gen. nov., sp. nov., a halophilic and alkaliphilic marine lactic acid bacterium isolated from marine organisms in temperate and subtropical areas of Japan. Int J Syst Evol Microbiol 53, 711–720.[CrossRef] [Google Scholar]
  12. Ishikawa, M., Nakajima, K., Itamiya, Y., Furukawa, S., Yamamoto, Y. & Yamasato, K.(2005).Halolactibacillus halophilus gen. nov., sp. nov. and Halolactibacillus miurensis sp. nov., halophilic and alkaliphilic marine lactic acid bacteria constituting a phylogenetic lineage in Bacillus rRNA group 1. Int J Syst Evol Microbiol 55, 2427–2439.[CrossRef] [Google Scholar]
  13. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. III. Edited by H. N. Munro. New York: Academic Press.
  14. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  15. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  16. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  17. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
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vol. , part 9, pp. 2169 - 2173

Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences. [PDF](15 KB)



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