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Volume 58, Issue 4

sp. nov., isolated from diseased sole ( Kaup) Free

Abstract

A novel Gram-negative, rod-shaped, gliding bacterial strain designated LL04 12.1.7 was isolated from diseased sole ( Kaup) in Galicia, Spain. Colonies were yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G+C content of the strain was 29.8 mol%. 16S rRNA gene sequence similarity analysis indicated that strain LL04 12.1.7 is a member of the genus in the family . Sequence similarities between the isolate and the type strains of other members of the genus were 96.7–94.8 %. The major fatty acids (>10 % of total fatty acids) were iso-C (23.1 %), iso-C 3-OH (10.6 %), C 6 (12.2 %) and summed feature 3 (comprising C 7 and/or iso-C 2-OH, 11.0 %). Genotypic and phenotypic data distinguished strain LL04 12.1.7 from the 11 recognized species, indicating that it represents a novel species, for which the name sp. nov. is proposed. The type strain is strain LL04 12.1.7 (=CECT 7292 =NCIMB 14368).

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2008-04-01
2024-04-16
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References

  1. Bernardet, J.-F., Segers, P., Vancanneyt, M., Berthe, F., Kersters, K. & Vandamme, P.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). Int J Syst Bacteriol 46, 128–148.[CrossRef] [Google Scholar]
  2. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  3. Buck, J. D.(1982). Non-staining (KOH) method for determination of gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  4. Choi, D. H., Kim, Y.-G., Hwang, C. Y., Yi, H., Chun, J. & Cho, B. C.(2006).Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56, 635–640.[CrossRef] [Google Scholar]
  5. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  6. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  7. Felsenstein, F.(2007).phylip (phylogeny inference package) version 3.67. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  8. Fitch, W. M.(1971). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef] [Google Scholar]
  9. Frette, L., Jørgensen, N. O. G., Irming, H. & Kroer, N.(2004).Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54, 519–524.[CrossRef] [Google Scholar]
  10. Hansen, G. H., Bergh, Ø., Michaelsen, J. & Knappskog, D.(1992).Flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of Atlantic halibut, Hippoglossus hippoglossus L. Int J Syst Bacteriol 42, 451–458.[CrossRef] [Google Scholar]
  11. Jung, S.-Y., Oh, T.-K. & Yoon, J.-H.(2006).Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56, 1577–1581.[CrossRef] [Google Scholar]
  12. Kimura, M.(1980). A simple model for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  13. Pazos, F., Santos, Y., Macías, A. R., Nuñez, S. & Toranzo, A. E.(1996). Evaluation of media for the successful culture of Flexibacter maritimus. J Fish Dis 19, 193–197.[CrossRef] [Google Scholar]
  14. Piñeiro-Vidal, M., Riaza, A. & Santos, Y.(2008).Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. Int J Syst Evol Microbiol 58, 21–25.[CrossRef] [Google Scholar]
  15. Reichenbach, H.(1989). Order I. Cytophagales Leadbetter 1974. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2011–2013. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  16. Reichenbach, H.(1992a). The order Cytophagales. In The Prokaryotes, 2nd edn, vol. 4, pp. 3631–3675. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  17. Reichenbach, H.(1992b).Flavobacteriaceae fam. nov. In Validation of the Publication of the New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 41. Int J Syst Bacteriol42, 327–329.
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI.
  20. Sheu, S.-Y., Lin, K.-Y., Chou, J.-H., Chang, P.-S., Arun, A. B., Young, C.-C. & Chen, W.-M.(2007).Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. Int J Syst Evol Microbiol 57, 1148–1153.[CrossRef] [Google Scholar]
  21. Stackebrandt, E. & Ebers, J.(2006). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155. [Google Scholar]
  22. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  23. Suzuki, M., Nakagawa, Y., Harayama, S. & Yamamoto, S.(2001). Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum. Int J Syst Evol Microbiol 51, 1639–1652.[CrossRef] [Google Scholar]
  24. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  25. Wakabayashi, H., Hikida, M. & Masumura, K.(1986).Flexibacter maritimus sp. nov., a pathogen of marine fishes. Int J Syst Bacteriol 36, 396–398.[CrossRef] [Google Scholar]
  26. Yoon, J.-H., Kang, S.-J. & Oh, T.-K.(2005).Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 793–798.[CrossRef] [Google Scholar]
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Tenacibaculum soleae sp. nov., isolated from diseased sole (Solea senegalensis Kaup)
Int J Syst Evol Microbiol 58, 881 (2008); https://doi.org/10.1099/ijs.0.65539-0
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