1887

Abstract

This proposal is our response to the recommendation of the International Committee on Systematics of Prokaryotes (Subcommittee on the taxonomy of ) that we ‘write a proposal to classify and ovine/caprine serogroup 11 as a single species’. Physiological and phylogenetic comparisons between 27 strains of and serogroup 11 showed that (i) growth and patterns of organic acid substrate use completely overlapped among strains; (ii) all had lipase and phosphatase activities; (iii) the strains were indistinguishable in their SDS–PAGE whole-cell protein profiles, which differed from five other species; (iv) strains were indistinguishable in immunoblotting of cell proteins and cross-reactivity in ELISA, but differed from other species; (v) DNA–DNA hybridization did not distinguish between the two groups, and (vi) comparison of 16S and 23S rRNA gene sequences of ten strains of serogroup 11 and six strains of showed that they shared 98–100 % similarity across all strains tested, but only 86–95 % to other species. Strains of the ovine/caprine serogroup 11 must therefore be reassigned as .

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65553-0
2008-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/1/308.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65553-0&mimeType=html&fmt=ahah

References

  1. Afshar, A., Stuart, P. & Huck, R. A.(1966). Granular vulvovaginitis (nodular venereal disease) of cattle associated with Mycoplasma bovigenitalium. Vet Rec 78, 512–519.[CrossRef] [Google Scholar]
  2. Al-Aubaidi, J. M., Dardiri, A. H. & Fabricant, J.(1972). Biochemical characterization and antigenic relationship of Mycoplasma mycoides subsp. mycoides Freundt and Mycoplasma mycoides subsp. capri (Edward) Freundt. Int J Syst Bacteriol 22, 155–164.[CrossRef] [Google Scholar]
  3. Bradbury, J. M.(2007). International Committee on Systematics of Prokaryotes. Subcommittee on the taxonomy of Mollicutes. Minutes of the meetings, 11 and 16 July 2004, Athens, GA, USA. Int J Syst Evol Microbiol 57, 187–191.[CrossRef] [Google Scholar]
  4. Brown, D. R., McLaughlin, G. S. & Brown, M. B.(1995). Taxonomy of the feline mycoplasmas Mycoplasma felifaucium, Mycoplasma feliminutum, Mycoplasma felis, Mycoplasma gatae, Mycoplasma leocaptivus, Mycoplasma leopharyngis, and Mycoplasma simbae by 16S rRNA gene sequence comparisons. Int J Syst Bacteriol 45, 560–564.[CrossRef] [Google Scholar]
  5. Carmichael, L. E., St George, T. D., Sullivan, N. D. & Horsfall, N.(1972). Isolation, propagation and characterization studies of an ovine mycoplasma responsible for proliferative interstitial pneumonia. Cornell Vet 62, 654–679. [Google Scholar]
  6. Chima, J. C., Erno, H. & Ojo, M. O.(1986). Characterisation and identification of caprine genital mycoplasmas. Acta Vet Scand 27, 531–539. [Google Scholar]
  7. Cottew, G. S., Lloyd, L. C., Pasonan, I. M. & Hoare, D. E.(1974). Isolation of a mycoplasma from vulvovaginitis in sheep. Aust Vet J 50, 576[CrossRef] [Google Scholar]
  8. Edward, D. G. & Freundt, E. A.(1956). The classification and nomenclature of organisms of the pleuropneumonia group. J Gen Microbiol 14, 197–207.[CrossRef] [Google Scholar]
  9. Erno, H., Al-Aubaidi, J. M., Ojo, M. O., Minga, U. M. & Sikdar, A.(1978). Classification and identification of ovine and caprine mycoplasmas. Acta Vet Scand 19, 392–406. [Google Scholar]
  10. Freundt, E. A.(1955). The classification of the pleuropneumonia-like group of organisms (Borrelomycetales). Int Bull Bacteriol Nomencl Taxon 5, 67–78. [Google Scholar]
  11. Gasparich, G. E., Whitcomb, R. F., Dodge, D., French, F. E., Glass, J. & Williamson, D. L.(2004). The genus Spiroplasma and its non-helical descendants: phylogenetic classification, correlation with phenotype and roots of the Mycoplasma mycoides clade. Int J Syst Evol Microbiol 54, 893–918.[CrossRef] [Google Scholar]
  12. Gourlay, R. N. & Howard, C. J.(1983). Recovery and identification of bovine mycoplasmas. In Methods in Mycoplasmology, vol. 2, pp. 86. Edited by J. G. Tully & S. Razin. London: Academic Press.
  13. Heldtander, M., Pettersson, B., Tully, J. G. & Johansson, K.-E.(1998). Sequences of the 16S rRNA genes and phylogeny of the goat mycoplasmas Mycoplasma adleri, Mycoplasma auris, Mycoplasma cottewii and Mycoplasma yeatsi. Int J Syst Bacteriol 48, 263–268.[CrossRef] [Google Scholar]
  14. Jackson, G. & Boughton, E.(1991). A mild outbreak of bovine mastitis associated with Mycoplasma bovigenitalium. Vet Rec 129, 444–446.[CrossRef] [Google Scholar]
  15. Johansson, R.-E. & Bradbury, J. M.(2007). Minute 15. International Committee on Systematics of Prokaryotes. Subcommittee on the taxonomy of Mollicutes. Minutes of the meetings, 7 and 12 July 2002, Vienna, Austria. Int J Syst Evol Microbiol 57, 184 [Google Scholar]
  16. Jurmanová, K., Hájková, M., Cerná, J. & Jiránek, E.(1978). The course of mastitides in cows infected into the mammary gland with strains of Mycoplasma bovigenitalium and M. bovis. Vet Med (Praha) 23, 587–596. [Google Scholar]
  17. Kumar, D., Gupta, P. P., Rana, J. S. & Banga, H. S.(1993). Pathology of Mycoplasma serogroup 11 induced mastitis in sheep. Aust Vet J 70, 70–71.[CrossRef] [Google Scholar]
  18. Laemmli, U. K.(1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef] [Google Scholar]
  19. Lin, Y.-C., Miles, R. J., Nicholas, R. A. J. & Wood, A. P.(2006). A rapid chromogenic microtitre assay of arginine aminopeptidase activity in Mycoplasma strains. Syst Appl Microbiol 29, 589–592.[CrossRef] [Google Scholar]
  20. Livingston, C. W. & Gauer, B. B.(1983). Occurrence of Mycoplasma sp. (2D) in Texas sheep flocks. Am J Vet Res 44, 868–869. [Google Scholar]
  21. Mackie, D. P. & Ball, H. J.(1984). Pathogenic variation of three strains of Mycoplasma bovigenitalium for the bovine mammary gland. Vet Rec 114, 456–457.[CrossRef] [Google Scholar]
  22. Nicholas, R. A. J., Wessels, M., Orme, P. K., Wood, E. & Sachse, K.(1999). Isolation of Mycoplasma ovine/caprine serogroup 11 from infertile sheep in Britain. Vet Rec 145, 434–435.[CrossRef] [Google Scholar]
  23. Nicholas, R. A. J., Khan, L., Houshaymi, B., Miles, R. J., Ayling, R. D., Hotzel, H. & Sachse, K.(2002). Close genetic and phenotypic relatedness between Mycoplasma ovine/caprine serogroup 11 and Mycoplasma bovigenitalium. Syst Appl Microbiol 25, 396–402.[CrossRef] [Google Scholar]
  24. Parham, K., Churchward, C. P., McAuliffe, L., Nicholas, R. A. J. & Ayling, R. D.(2006). A high level of strain variation within the Mycoplasma ovipneumoniae population of the UK has significant implications for disease diagnosis and management. Vet Microbiol 118, 83–90.[CrossRef] [Google Scholar]
  25. Pettersson, B., Uhlén, M. & Johansson, K.-E.(1996). Phylogeny of some mycoplasmas from ruminants based on 16S rRNA sequences and definition of a new cluster within the hominis group. Int J Syst Bacteriol 46, 1093–1098.[CrossRef] [Google Scholar]
  26. Pitcher, D. G. & Nicholas, R. A. J.(2005). Mycoplasma host specificity: fact or fiction? Vet J 170, 300–306.[CrossRef] [Google Scholar]
  27. Poumarat, F., Longchambon, D. & Martel, J. L.(1992). Application of dot immunoblotting on membrane filtration (MF dot) to the study of relationships with M. mycoides cluster and within “glucose and arginine negative cluster” of ruminant mycoplasmas. Vet Microbiol 32, 375–390.[CrossRef] [Google Scholar]
  28. Prasad, L. N., Gupta, P. P. & Singh, N.(1984). Isolation of mycoplasmas from goat mastitis. Indian J Anim Sci 54, 1172–1174. [Google Scholar]
  29. Rana, J. S., Gupta, P. P. & Ahuja, S. P.(1993). Biochemical changes of the milk in experimental caprine mastitis induced by Mycoplasma serogroup 11 (2-D). Acta Vet Hung 41, 139–149. [Google Scholar]
  30. Razin, S. & Freundt, E. A.(1984). The mycoplasmas. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 740–793. Edited by N. R. Krieg. Baltimore: Williams & Wilkins.
  31. Ruffin, D. C.(2001). Mycoplasma infections in small ruminants. Vet Clin North Am Food Anim Pract 17, 315–332. [Google Scholar]
  32. Sachse, K., Diller, R. & Hotzel, H.(2002). A signature region in the 23S rRNA gene characteristic for Mycoplasma and Acholeplasma spp. harbours target sequences for identification of species, genus and clusters, as well as for distinction of mollicutes from other bacteria. In Abstracts of the14th International Congress of the International Organization for Mycoplasmology (IOM), Vienna, Austria, 7–12 July 2002, pp. 174–175.
  33. Stakenborg, T., Vicca, J., Butaye, P., Maes, D., De Baere, T., Verhelst, R., Peeters, J., De Kruf, A., Haesebrouck, F. & Vaneechoutte, M.(2005). Evaluation of amplified rDNA restriction analysis (ARDRA) for the identification of Mycoplasma species. BMC Infect Dis 5, 46[CrossRef] [Google Scholar]
  34. Tasker, S., Helps, C. R., Day, M. J., Gruffydd-Jones, T. J. & Harbour, D. A.(2003). Use of real-time PCR to detect and quantify Mycoplasma haemofelis and ‘Candidatus Mycoplasma haemominutum’ DNA. J Clin Microbiol 41, 439–441.[CrossRef] [Google Scholar]
  35. Thiede, S., Spergser, J., Rosengarten, R., Jakob, W., Streich, W. J., Krasinska, M. & Frölich, K.(2002). Antibodies against Mycoplasma bovigenitalium in free-living European bison (Bison bonasus) with balanoposthitis. J Wildl Dis 38, 760–763.[CrossRef] [Google Scholar]
  36. Weisburg, W. G., Tully, J. G., Rose, D. L., Petzel, J. P., Oyaizu, H., Yang, D., Mandelco, L., Sechrest, J., Lawrence, T. G. & Van Etten, J.(1989). A phylogenetic analysis of the mycoplasmas: basis for their classification. J Bacteriol 171, 6455–6467. [Google Scholar]
  37. Woubit, S., Lorenzon, S., Peyraud, A., Manso-Silvan, L. & Thiaucourt, F.(2004). A specific PCR for the identification of Mycoplasma capricolum subsp. capripneumoniae, the causative agent of contagious caprine pleuropneumonia (CCPP). Vet Microbiol 104, 125–132.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65553-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65553-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 308 - 312

Comparative 16S and 23S rRNA gene sequence similarities between strain 2-D of the serogroup 11 and other strains of serogroup 11 and of .

Comparative 16S rRNA gene sequence similarities of other species of to the type strain of and to serogroup 11 strain 2-D.



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error