RT Journal Article SR Electronic(1) A1 Peng, Guixiang A1 Yuan, Qinghua A1 Li, Huaxing A1 Zhang, Wu A1 Tan, ZhiyuanYR 2008 T1 Rhizobium oryzae sp. nov., isolated from the wild rice Oryza alta JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 9 SP 2158 OP 2163 DO https://doi.org/10.1099/ijs.0.65632-0 PB Microbiology Society, SN 1466-5034, AB During a study of endophytic nitrogen-fixing bacteria present in the wild rice species Oryza alta, eight novel isolates were obtained from surface-sterilized roots and classified in the genus Rhizobium on the basis of almost-complete 16S rRNA gene sequence analysis. These strains can nodulate Phaseolus vulgaris and Glycine max. The highly similar protein patterns, DNA fingerprint patterns of insertion sequence-based PCR (IS-PCR) and DNA–DNA hybridizations showed that these novel isolates were members of the same species. The closest phylogenetic relatives of the representative strain Alt 505T of the novel group were Rhizobium etli CFN 42T and Rhizobium indigoferae CCBAU 71714T, with 96.2 and 96.0 % 16S rRNA gene sequence similarity, respectively. Low DNA–DNA relatedness with the type strains of R. etli, R. indigoferae, Rhizobium hainanense, Rhizobium mongolense and Rhizobium galegae and differences in IS-PCR fingerprinting patterns, SDS-PAGE of proteins, antibiotic resistance, phenotypic tests and comparison of cellular fatty acids with Rhizobium species indicated that the novel group of isolates were distinct from previously described species. Based on these results, we propose to place them in a novel species, as Rhizobium oryzae sp. nov. The type strain is Alt 505T (=LMG 24253T =CGMCC 1.7048T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65632-0