RT Journal Article SR Electronic(1) A1 Vanlaere, Elke A1 LiPuma, John J. A1 Baldwin, Adam A1 Henry, Deborah A1 De Brandt, Evie A1 Mahenthiralingam, Eshwar A1 Speert, David A1 Dowson, Chris A1 Vandamme, PeterYR 2008 T1 Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 7 SP 1580 OP 1590 DO https://doi.org/10.1099/ijs.0.65634-0 PB Microbiology Society, SN 1466-5034, AB The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach. The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA–DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3T =LMG 24064T =CCUG 54555T), Burkholderia diffusa sp. nov. (type strain AU1075T =LMG 24065T =CCUG 54558T), Burkholderia arboris sp. nov. (type strain ES0263AT =LMG 24066T =CCUG 54561T), Burkholderia seminalis sp. nov. (type strain AU0475T =LMG 24067T =CCUG 54564T) and Burkholderia metallica sp. nov. (type strain AU0553T =LMG 24068T =CCUG 54567T). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65634-0