1887

Abstract

A taxonomic study was performed on strain MSL-08, which was isolated from a soil sample collected from Bigeum Island. The novel isolate was aerobic and Gram-positive. Cells were short and motile rods. Growth temperature ranged from 20 to 28 °C and the pH for growth ranged from 6.5 to 12.0. The optimum growth temperature and pH were 28 °C and 7.3, respectively. The predominant menaquinone was MK-9. Cell wall analysis showed B-type peptidoglycan containing 2,4-diaminobutyric acid, alanine, glycine, glutamate and lysine. The diagnostic phospholipids were diphosphatidylglycerol and phosphatidylglycerol. The major fatty acids were ai-C, i-C, C 7 and ai-C. The DNA G+C content was 67.5 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain MSL-08 had less than 97 % similarity to any recognized species of the genus . DSM 10309 was found to be the closest neighbour (96.95 %) to the novel strain. Based on the 16S rRNA gene sequence analysis and phenotypic characteristics, it is proposed that strain MSL-08 represents a novel member of the genus for which the name sp. nov. is proposed. The type strain is MSL-08 (=DSM 19442=KCTC 19311).

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2008-08-01
2024-04-18
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References

  1. Cerny, G.(1978). Studies on the aminopeptidase test for the distinction of gram-negative from gram-positive bacteria. Appl Microbiol Biotechnol 5, 113–122.[CrossRef] [Google Scholar]
  2. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y. W.(2007). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequence. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef] [Google Scholar]
  3. Cui, X. L., Mao, P. H., Tseng, M., Li, W. J., Zhang, L. P., Xu, L. H. & Jiang, C. L.(2001).Streptomonospora gen. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363. [Google Scholar]
  4. Felsenstein, J.(1981). Evolutionary tree from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  5. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  6. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef] [Google Scholar]
  7. Kämpfer, P., Rainey, F. A., Anderson, M. A., Nurmiaho Lassila, E.-L., Ulrych, U., Busse, H.-J., Weiss, N., Mikkola, R. & Salkinoja-Salonen, M.(2000).Frigoribacterium faeni gen. nov., sp. nov., a novel psychrophilic genus of the family Microbacteriaceae. Int J Syst Evol Microbiol 50, 355–363.[CrossRef] [Google Scholar]
  8. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  9. Kimura, M.(1983).The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  10. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  11. Kroppenstedt, R. M.(1982). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2367.[CrossRef] [Google Scholar]
  12. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  13. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  14. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  15. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  16. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC News Lett 20, 16 [Google Scholar]
  17. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  18. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  19. Tang, S. K., Li, W. J., Dong, W., Zhang, Y. G., Xu, L. H. & Jiang, C. L.(2003). Studies of the biological characteristics of some halophilic and halotolerant actinomycetes isolated from saline and alkaline soils. Actinomycetologica 17, 6–10.[CrossRef] [Google Scholar]
  20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24, 4876–4882. [Google Scholar]
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