1887

Abstract

A novel mesophilic, Gram-positive, cream- to pale yellow-pigmented, aerobic bacterium, designated MSL-15, was isolated from a soil sample collected from Bigeum Island, South Korea. The cells were catalase-positive, motile and irregular rods. The diamino acid content of the cell-wall peptidoglycan was determined to be 2,4-diaminobutyric acid. Strain MSL-15 grew well between 20 and 28 °C, with optimum growth occurring at 25–28 °C. The major fatty acids were anteiso-C, iso-C, anteiso-C and iso-C. The genomic DNA G+C content was 70.0 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MSL-15 was associated with the genus and exhibited 96.6 % sequence similarity to JCM 13925 and 94.8 % similarity to JCM 1463. Phenotypic, chemotaxonomic and phylogenetic data revealed that strain MSL-15 represents a novel species within the genus , for which the name is proposed. The type strain is MSL-15 (=DSM 19267=KCTC 19270).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65650-0
2008-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/5/1241.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65650-0&mimeType=html&fmt=ahah

References

  1. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  2. Cui, X. L., Mao, P. H., Tseng, M., Li, W. J., Zhang, L. P., Xu, L. H. & Jiang, C. L.(2001).Streptomonospora gen. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363. [Google Scholar]
  3. Felsenstein, J.(1981). Evolutionary tree from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  5. Fitch, W. M. & Margoliash, E.(1967). Construction of phylogenetic trees: a method based on mutation distances as estimated from cytochrome c sequences is of general applicability. Science 155, 279–284.[CrossRef] [Google Scholar]
  6. Inoue, K.(1976). Quantitative ecology of microorganisms of Showa Station in Antarctica and isolation of psychrophiles. J Gen Appl Microbiol 22, 143–150.[CrossRef] [Google Scholar]
  7. Inoue, K. & Komagata, K.(1976). Taxonomic study on obligately psychrophilic bacteria isolated from Antarctica. J Gen Appl Microbiol 22, 165–176.[CrossRef] [Google Scholar]
  8. Kämpfer, P., Kroppenstedt, R. M. & Dott, W.(1991). A numerical classification of the genera Streptomyces and Streptoverticillium using miniaturized physiological tests. J Gen Microbiol 137, 1831–1891.[CrossRef] [Google Scholar]
  9. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  10. Kimura, M.(1983).The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  11. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  12. Kroppenstedt, R. M.(1982). Separation of bacterial menaquinones by HPLC using reverse phase (RP 18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2367.[CrossRef] [Google Scholar]
  13. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  14. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  16. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  17. Reasoner, D. J. & Geldreich, E. E.(1985). A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49, 1–7. [Google Scholar]
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 1–6. [Google Scholar]
  20. Suzuki, K., Sasaki, J., Uramoto, M., Nakase, T. & Komagata, K.(1997).Cryobacterium psychrophilum gen. nov., sp. nov., nom. rev., comb. nov., an obligately psychrophilic actinomycete to accommodate ‘‘Curtobacterium psychrophilum’’ Inoue and Komagata 1976. Int J Syst Bacteriol 47, 474–478.[CrossRef] [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  22. Zhang, D.-C., Wang, H.-X., Cui, H.-L., Yang, Y., Li, H.-C., Dong, X.-Z. & Zhou, P.-J.(2007).Cryobacterium psychrotolerans sp. nov., a novel psychrotolerant bacterium isolated from the China No.1 glacier. Int J Syst Evol Microbiol 57, 866–869.[CrossRef] [Google Scholar]
  23. Zlamala, C., Schumann, P., Kämpfer, P., Rosselló-Mora, R., Lubitz, W. & Busse, H.-J.(2002).Agrococcus baldri sp. nov., isolated from the air in the ‘Virgilkapelle’ in Vienna. Int J Syst Evol Microbiol 52, 1211–1216.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65650-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65650-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error