1887

Abstract

A Gram-positive, aerobic, non-motile actinobacterium strain, designated YIM 45607, was isolated from a rhizosphere soil sample in Kunming, south-west China. Chemotaxonomically, the isolate contained chemical markers that supported its assignment to the genus . On the basis of 16S rRNA gene sequence similarity analysis, strain YIM 45607 formed a new subline within the genus , with as its closest phylogenetic neighbour (98.2 % 16S rRNA gene sequence similarity to the type strain). However, DNA–DNA hybridization demonstrated that strain YIM 45607 was different from DSM 20307 (35.4 % relatedness). Based on polyphasic analysis, strain YIM 45607 could be clearly distinguished from other species of the genus . The isolate therefore represents a novel species of , for which the name sp. nov. is proposed. The type strain is strain YIM 45607 (=KCTC 19149 =DSM 45001).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65673-0
2008-06-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/6/1467.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65673-0&mimeType=html&fmt=ahah

References

  1. Buck, J. D.(1982). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44, 992–993. [Google Scholar]
  2. Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef] [Google Scholar]
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  5. Goodfellow, M. & Pirouz, T.(1982). Numerical classification of sporoactinomycetes containing meso-diaminopimelic acid in the cell wall. J Gen Microbiol 128, 503–527. [Google Scholar]
  6. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  7. Groth, I., Rodríguez, C., Schütze, B., Schmitz, P., Leistner, E. & Goodfellow, M.(2004). Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov. Int J Syst Evol Microbiol 54, 2121–2129.[CrossRef] [Google Scholar]
  8. He, L., Li, W., Huang, Y., Wang, L. M., Liu, Z. H., Lanoot, B. J., Vancanneyt, M. & Swings, J.(2005).Streptomyces jietaisiensis sp. nov., isolated from soil in northern China. Int J Syst Evol Microbiol 55, 1939–1944.[CrossRef] [Google Scholar]
  9. Kelly, K. L.(1964).Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  10. Kimura, M.(1983).TheNeutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  11. Kovacs, N.(1956). Identification of Pseudomonas pyocyanea by oxidase reaction. Nature 178, 703–704. [Google Scholar]
  12. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  13. Lechevalier, M. P. & Lechevalier, H. A.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  14. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A.(1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef] [Google Scholar]
  15. Li, W.-J., Xu, P., Schumann, P., Zhang, Y.-Q., Pukall, R., Xu, L.-H., Stackebrandt, E. & Jiang, C.-L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef] [Google Scholar]
  16. McMinn, E. J., Alderson, G., Dodson, H. I., Goodfellow, M. & Ward, A. C.(2000). Genomic and phenomic differentiation of Rhodococcus equi and related strains. Antonie van Leeuwenhoek 78, 331–340.[CrossRef] [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  18. Minnikin, D. E., Alshamaony, L. & Goodfellow, M.(1975). Differentiation of Mycobacterium, Nocardia, and related taxa by thin-layer chromatographic analysis of whole-organism methanolysates. J Gen Microbiol 88, 200–204.[CrossRef] [Google Scholar]
  19. Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef] [Google Scholar]
  20. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  21. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  22. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  23. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L.(1997). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef] [Google Scholar]
  24. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  25. Suzuki, S., Okuda, T. & Komatsubara, S.(1999). Selective isolation and distribution of Sporichthya strains in soil. Appl Environ Microbiol 65, 1930–1935. [Google Scholar]
  26. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H.(1983). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54, 31–36.[CrossRef] [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  28. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  29. Yassin, A. F.(2005).Rhodococcus triatomae sp. nov., isolated from a blood-sucking bug. Int J Syst Evol Microbiol 55, 1575–1579.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65673-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65673-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error