1887

Abstract

Nine bacterial strains were studied by means of rep-PCR, 16S rRNA gene sequence analysis, DNA–DNA hybridization and physiological characterization. Typing analysis by means of rep-PCR showed that all nine strains were highly homogeneous, with similarities above 94 %. The strains were isolated from the same geographical area (Mazatlán, Sinaloa state, Mexico) and the same type of host (cultured rose snapper, ), although from different individuals and organs. Comparison of the almost-complete 16S rRNA gene sequences of five strains showed that they belonged to the genus and are closely related to the type strains of and , with similarity values ranging from 97.9 to 98.1 % and from 97.4 to 97.8 %, respectively. The DNA–DNA hybridization value of strain CAIM 797 with the type strain of (CAIM 495) was 47.5 %, with a reciprocal value of 44.7 %. The main phenotypic features of the strains were in agreement with the phylogenetic and genomic data. The results presented here support the description of a novel species, for which the name sp. nov. is proposed, with strain CAIM 797 (=CECT 7298) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65719-0
2008-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/58/7/1621.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65719-0&mimeType=html&fmt=ahah

References

  1. Alsina, M. & Blanch, A. R.(1994). A set of keys for biochemical identification of environmental Vibrio species. J Appl Bacteriol 76, 79–85.[CrossRef] [Google Scholar]
  2. Cabanillas-Beltran, H., Llausas-Magana, E., Romero, R., Espinoza, A., Garcia-Gasca, A., Nishibuchi, M., Ishibashi, M. & Gomez-Gil, B.(2006). Outbreak of gastroenteritis caused by the pandemic Vibrio parahaemolyticus O3 : K6 in Mexico. FEMS Microbiol Lett 265, 76–80.[CrossRef] [Google Scholar]
  3. Euzéby, J. P.(1997). List of bacterial names with standing in nomenclature: a folder available on the internet. Int J Syst Bacteriol 47, 590–592.[CrossRef] [Google Scholar]
  4. Figueras, M. J., Alperi, A., Guarro, J. & Martinez-Murcia, A. J.(2006). Genotyping of isolates included in the description of a novel species should be mandatory. Int J Syst Evol Microbiol 56, 1183–1184.[CrossRef] [Google Scholar]
  5. Gomez-Gil, B., Thompson, F. L., Thompson, C. C. & Swings, J.(2003).Vibrio pacinii sp. nov., from cultured aquatic organisms. Int J Syst Evol Microbiol 53, 1569–1573.[CrossRef] [Google Scholar]
  6. Gomez-Gil, B., Fajer-Avila, E. & Garcia-Vargas, F.(2007). Vibrios of the spotted rose snapper Lutjanus guttatus Steindachner, 1869 from northwestern Mexico. J Appl Microbiol 102, 1518–1526.[CrossRef] [Google Scholar]
  7. Kosman, E. & Leonard, K. J.(2005). Similarity coefficients for molecular markers in studies of genetic relationships between individuals for haploid, diploid, and polyploid species. Mol Ecol 14, 415–424.[CrossRef] [Google Scholar]
  8. Kumar, S., Tamura, K., Jakobsen, I.-B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  9. Lightner, D. V. & Redman, R. M.(1998). Shrimp diseases and current diagnostic methods. Aquaculture 164, 201–220.[CrossRef] [Google Scholar]
  10. Macián, M. C., Ludwig, W., Aznar, R., Grimont, P. A. D., Schleifer, K. H., Garay, E. & Pujalte, M. J.(2001).Vibrio lentus sp. nov., isolated from Mediterranean oysters. Int J Syst Evol Microbiol 51, 1449–1456. [Google Scholar]
  11. Macián, M. C., Garay, E., Grimont, P. A. D. & Pujalte, M. J.(2004).Vibrio ponticus sp. nov., a neighbour of V. fluvialis-V. furnissii clade, isolated from gilthead sea bream, mussels and seawater. Syst Appl Microbiol 27, 535–540.[CrossRef] [Google Scholar]
  12. Soto-Rodriguez, S. A., Roque, A., Lizarraga-Partida, M. L., Guerra-Flores, A. L. & Gomez-Gil, B.(2003). Virulence of luminous vibrios to Artemia franciscana nauplii. Dis Aquat Organ 53, 231–240.[CrossRef] [Google Scholar]
  13. Thompson, F. L., Li, Y., Gomez-Gil, B., Thompson, C. C., Hoste, B., Vandemeulebroecke, K., Rupp, G. S., Pereira, A., De Bem, M. M. & other authors(2003a).Vibrio neptunius sp. nov., Vibrio brasiliensis sp. nov. and Vibrio xuii sp. nov., isolated from the marine aquaculture environment (bivalves, fish, rotifers and shrimps). Int J Syst Evol Microbiol 53, 245–252.[CrossRef] [Google Scholar]
  14. Thompson, F. L., Thompson, C. C., Hoste, B., Vandemeulebroecke, K., Gullian, M. & Swings, J.(2003b).Vibrio fortis sp. nov. and Vibrio hepatarius sp. nov., isolated from aquatic animals and the marine environment. Int J Syst Evol Microbiol 53, 1495–1501.[CrossRef] [Google Scholar]
  15. Thompson, F. L., Iida, T. & Swings, J.(2004). Biodiversity of vibrios. Microbiol Mol Biol Rev 68, 403–431.[CrossRef] [Google Scholar]
  16. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  17. Ziemke, F., Höfle, M. G., Lalucat, J. & Rosselló-Mora, R.(1998). Reclassification of Shewanella putrefaciens Owen's genomic group II as Shewanella baltica sp. nov. Int J Syst Bacteriol 48, 179–186.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65719-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65719-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error