1887

Abstract

A Gram-negative, aerobic, slightly halophilic, rod-shaped bacterium was isolated from black sand in Soesoggak, Jeju island, Korea. The strain, designated J63, was oxidase- and catalase-positive and arginine dihydrolase-negative. The isolate required Na for growth and differed from phenotypically related species by being able to utilize sucrose and -galactose as a carbon source. Phylogenetic analysis based on the sequence of the 16S rRNA gene revealed that strain J63 belongs to the genus . It exhibited 16S rRNA gene sequence similarities of 97.6–98.7 % to the closely related species , , and . The phylogenetic analysis revealed that strain J63 comprised a relatively long subline of descent, shared a branch point with the outlying species and occupied a phylogenetically distant position on the main branch. Based on DNA–DNA hybridization, the levels of relatedness between strain J63 and NBRC 102224, CIP 108405 and JCM 20774 were 56.2, 45.1 and 51.3 %, respectively. On the basis of the phenotypic, genetic and phylogenetic data, strain J63 should be placed in the genus as representing a novel species, for which the name sp. nov. is proposed. The type strain is J63 (=KCTC 22118=JCM 14948).

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2008-12-01
2024-03-29
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References

  1. Baumann, L., Baumann, P., Mandel, M. & Allen, R. D.(1972). Taxonomy of aerobic marine eubacteria. J Bacteriol 110, 402–429. [Google Scholar]
  2. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  3. Gonzalez, J. M. & Saiz-Jimenez, C.(2002). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ Microbiol 4, 770–773.[CrossRef] [Google Scholar]
  4. Gordon, R. E., Haynes, W. C. & Pang, C. H.-N.(1973).The Genus Bacillus. US Department of Agriculture Handbook no. 427. Washington, DC: Agricultural Research Service.
  5. Gupta, P., Chaturvedi, P., Pradhan, S., Delille, D. & Shivaji, S.(2006).Marinomonas polaris sp. nov., a psychrohalotolerant strain isolated from coastal sea water of the subantarctic Kerguelen islands. Int J Syst Evol Microbiol 56, 361–364.[CrossRef] [Google Scholar]
  6. Ivanova, E. P., Onyshchenko, O. M., Christen, R., Lysenko, A. M., Zhukova, N. V., Shevchenko, L. S. & Kiprianova, E. A.(2005).Marinomonas pontica sp. nov., isolated from the Black Sea. Int J Syst Evol Microbiol 55, 275–279.[CrossRef] [Google Scholar]
  7. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  8. Kluge, A. G. & Farris, F. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  9. Lau, K. W., Ren, J., Wai, N. L., Lau, S. C., Qian, P. Y., Wong, P. K. & Wu, M.(2006).Marinomonas ostreistagni sp. nov., isolated from a pearl-oyster culture pond in Sanya, Hainan Province, China. Int J Syst Evol Microbiol 56, 2271–2275.[CrossRef] [Google Scholar]
  10. Macian, M. C., Arahal, D. R., Garay, E. & Pujalte, M. J.(2005).Marinomonas aquamarina sp. nov., isolated from oysters and seawater. Syst Appl Microbiol 28, 145–150.[CrossRef] [Google Scholar]
  11. Prabagaran, S. R., Suresh, K., Manorama, R., Delille, D. & Shivaji, S.(2005).Marinomonas ushuaiensis sp. nov., isolated from coastal sea water in Ushuaia, Argentina, sub-Antarctica. Int J Syst Evol Microbiol 55, 309–313.[CrossRef] [Google Scholar]
  12. Roh, S. W., Sung, Y., Nam, Y. D., Chang, H. W., Kim, K. H., Yoon, J. H., Jeon, C. O., Oh, H. M. & Bae, J. W.(2008).Arthrobacter soli sp. nov., a novel bacterium isolated from wastewater reservoir sediment. J Microbiol 46, 40–44.[CrossRef] [Google Scholar]
  13. Romanenko, L. A., Uchino, M., Mikhailov, V. V., Zhukova, N. V. & Uchimura, T.(2003).Marinomonas primoryensis sp. nov., a novel psychrophile isolated from coastal sea-ice in the Sea of Japan. Int J Syst Evol Microbiol 53, 829–832.[CrossRef] [Google Scholar]
  14. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  15. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  16. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  17. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  18. Solano, F. & Sanchez-Amat, A.(1999). Studies on the phylogenetic relationships of melanogenic marine bacteria: proposal of Marinomonas mediterranea sp. nov. Int J Syst Bacteriol 49, 1241–1246.[CrossRef] [Google Scholar]
  19. Stackebrandt, E., Liesack, W. & Goebel, B. M.(1993). Bacterial diversity in a soil sample from a subtropical Australian environment as determined by 16S rDNA analysis. FASEB J 7, 232–236. [Google Scholar]
  20. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  21. Van Landschoot, A. & De Ley, J.(1983). Intra- and intergeneric similarities of the rRNA cistrons of Alteromonas, Marinomonas (gen. nov.) and some other Gram-negative bacteria. J Gen Microbiol 129, 3057–3074. [Google Scholar]
  22. Yoon, J. H., Kang, S. J. & Oh, T. K.(2005).Marinomonas dokdonensis sp. nov., isolated from sea water. Int J Syst Evol Microbiol 55, 2303–2307.[CrossRef] [Google Scholar]
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