1887

Abstract

Gram-positive, rod-shaped, non-motile lactic acid bacteria, strains iz4b-1, iz4b-2 and iz4c-1, were isolated in an attempt to study the composition of the flora of Japanese pickles. Analysis of their 16S rRNA gene sequences revealed that the strains clustered in the group, and comparatively high similarities were shown to ‘’ CECT 5920 (97.0 %), KU-3 (96.4 %) and LP33 (94.4 %). DNA–DNA hybridization assays clearly revealed that the isolates represented a novel taxon. The DNA G+C content was 40.6 mol% and the peptidoglycan type was -Lys–-Asp. Thus, these isolates represent a novel species, for which the name sp. nov. is proposed. The type strain is iz4b-1 (=DSM 19682 =JCM 14932).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.65772-0
2009-01-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/1/83.html?itemId=/content/journal/ijsem/10.1099/ijs.0.65772-0&mimeType=html&fmt=ahah

References

  1. Cai, Y., Okada, H., Mori, H., Benno, Y. & Nakase, T.(1999).Lactobacillus paralimentarius sp. nov., isolated from sourdough. Int J Syst Bacteriol 49, 1451–1455.[CrossRef] [Google Scholar]
  2. Chenoll, E., Macián, M. C. & Aznar, R.(2005).Lactobacillus tucceti sp. nov., a new lactic acid bacterium isolated from sausage. Syst Appl Microbiol 29, 389–395. [Google Scholar]
  3. Ehrmann, M. A., Müller, M. R. & Vogel, R. F.(2003). Molecular analysis of sourdough reveals Lactobacillus mindensis sp. nov. Int J Syst Evol Microbiol 53, 7–13.[CrossRef] [Google Scholar]
  4. Endo, A. & Okada, S.(2005).Lactobacillus satsumensis sp. nov., isolated from mashes of shochu, a traditional Japanese distilled spirit made from fermented rice and other starchy materials. Int J Syst Evol Microbiol 55, 83–85.[CrossRef] [Google Scholar]
  5. Ezaki, T., Hashimoto, Y., Takeuchi, N., Yamamoto, H., Liu, S.-L., Miura, H., Matsui, K. & Yabuuchi, E.(1988). Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. J Clin Microbiol 26, 1708–1713. [Google Scholar]
  6. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  7. Felis, G. E. & Dellaglio, F.(2007). Taxonomy of lactobacilli and bifidobacteria. Curr Issues Intest Microbiol 8, 44–61. [Google Scholar]
  8. Felsenstein, J.(1978). Cases in which parsimony and compatibility methods will be positively misleading. Syst Zool 27, 401–410.[CrossRef] [Google Scholar]
  9. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(2007).phylip (phylogeny inference package) version 3.67. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  11. Johnson, J. L.(1973). Use of nucleic-acid homologies in the taxonomy of anaerobic bacteria. Int J Syst Bacteriol 23, 308–315.[CrossRef] [Google Scholar]
  12. Kandler, O., Schillinger, U. & Weiss, N.(1983).Lactobacillus halotolerans sp. nov., nom. rev. and Lactobacillus minor sp. nov., nom. rev. Syst Appl Microbiol 4, 280–285.[CrossRef] [Google Scholar]
  13. Katayama-Fujimura, Y., Komatsu, Y., Kuraishi, H. & Kaneko, T.(1984). Estimation of DNA base composition by high performance liquid chromatography of its nuclease P1 hydrolysate. Agric Biol Chem 48, 3169–3172.[CrossRef] [Google Scholar]
  14. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  15. Kröckel, L., Schillinger, U., Franz, C. M. A. P., Bantleon, A. & Ludwig, W.(2003).Lactobacillus versmoldensis sp. nov., isolated from raw fermented sausage. Int J Syst Evol Microbiol 53, 513–517.[CrossRef] [Google Scholar]
  16. Pitcher, D. G., Saunders, N. A. & Owen, R. J.(1989). Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 8, 151–156.[CrossRef] [Google Scholar]
  17. Reuter, G.(1983).Lactobacillus alimentarius sp. nov., nom. rev. and Lactobacillus farciminis sp. nov., nom. rev. Syst Appl Microbiol 4, 277–279.[CrossRef] [Google Scholar]
  18. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  19. Scheirlinck, I., Van der Meulen, R., Van Schoor, A., Huys, G., Vandamme, P., De Vuyst, L. & Vancanneyt, M.(2007).Lactobacillus crustorum sp. nov., isolated from two traditional Belgian wheat sourdoughs. Int J Syst Evol Microbiol 57, 1461–1467.[CrossRef] [Google Scholar]
  20. Schleifer, K. H.(1985). Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18, 123–156. [Google Scholar]
  21. Schleifer, K. H. & Kandler, O.(1972). The peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  22. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  23. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  24. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  25. Valcheva, R., Ferchichi, M. F., Korakli, M., Ivanova, I., Gänzle, M. G., Vogel, R. F., Prévost, H., Onno, B. & Dousset, X.(2006).Lactobacillus nantensis sp. nov., isolated from French wheat sourdough. Int J Syst Evol Microbiol 56, 587–591.[CrossRef] [Google Scholar]
  26. Yoon, J. H., Kang, S. S., Mheen, T. I., Ahn, J. S., Lee, H. J., Kim, T. K., Park, C. S., Kho, Y. H., Kang, K. H. & Park, Y. H.(2000).Lactobacillus kimchii sp. nov., a new species from kimchi. Int J Syst Evol Microbiol 50, 1789–1795. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.65772-0
Loading
/content/journal/ijsem/10.1099/ijs.0.65772-0
Loading

Data & Media loading...

Supplements

vol. , part 1, pp. 83 - 86

Phylogenetic trees showing the relationships between sp. nov. iz4b-1 and related species on the basis of 16S rRNA gene sequences, constructed using the maximum-likelihood (S1) and maximum-parsimony (S2) methods. [PDF](666 KB)

DNA–DNA hybridization between sp. nov. strains iz4b-1 , iz4b-2 and iz4c-1 and ' ' CECT 5920

In each assay, each combination was tested three times and mean percentages are indicated. –, Not done.



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error