Halorubrum kocurii sp. nov., an archaeon isolated from a saline lake
Gutiérrez, M. C. and Castillo, A. M. and Pagaling, E. and Heaphy, S. and Kamekura, M. and Xue, Y. and Ma, Y. and Cowan, D. A. and Jones, B. E. and Grant, W. D. and Ventosa, A.,, 58, 2031-2035 (2008),
doi = https://doi.org/10.1099/ijs.0.65840-0,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A Gram-negative, non-motile, neutrophilic, rod-shaped, extremely halophilic archaeon, designated strain BG-1T, was isolated from a salt lake, Lake Bagaejinnor, in Inner Mongolia, China. Strain BG-1T was able to grow at 25–55 °C, required at least 2.5 M NaCl for growth (with an optimum at 3.4 M NaCl) and grew at pH 6.0–9.0 (with an optimum at pH 7.5). Hypotonic treatment with less than 2.0 M NaCl caused cell lysis. Phylogenetic analysis of the almost-complete 16S rRNA gene sequence positioned the isolate within the genus Halorubrum in the family Halobacteriaceae. Strain BG-1T was most closely related to Halorubrum aidingense 31-hongT (98.8 % sequence similarity), Halorubrum saccharovorum NCIMB 2081T (98.6 %), Halorubrum lacusprofundi ACAM 34T (98.6 %) and Halorubrum lipolyticum 9-3T (98.4 %). However, values for DNA–DNA hybridization between strain BG-1T and the most closely related members of the genus Halorubrum were below 40 %. Analysis of the polar lipids of strain BG-1T revealed the presence of mannosyl-2-sulfate-(1-4)-glycosyl-archaeol, the main glycolipid found in neutrophilic species of the genus Halorubrum. The G+C content of the genomic DNA was 69.4 mol% (T m). Comparison of the phenotypic characteristics of the strain with those of Halorubrum species supported the conclusion that BG-1T represents a novel species within this genus, for which the name Halorubrum kocurii sp. nov. is proposed. The type strain is BG-1T (=CECT 7322T =CGMCC 1.7018T =JCM 14978T).,
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