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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 58, Issue 9

Reclassification of as comb. nov., a butyrate-producing ruminal bacterium Free

Abstract

It is proposed that be reclassified as comb. nov. on the basis of phylogenetic position, DNA G+C content and physiological traits. Phylogenetic analyses based on 16S rRNA gene sequences from an extensive range of taxa within clostridial rRNA subcluster XIVa grouped together with isolates of the genus , though this species was genetically distinct from the extant species examined. The DNA G+C content of was originally erroneously reported as 28 mol%. However the genome sequence of the type strain of , strain B316, and HPLC analysis estimate the DNA G+C content as 40 mol%, which is within the range reported for strains of . was distinguishable from other species of the genus as the 16S rRNA gene from strain B316 shared less than 97 % sequence similarity with sequences from the type strains of species. was also able to convert linoleic acid to stearic acid, in contrast to other species of . Physiological characteristics, including carbon source utilization, volatile fatty acid production and proteinase activities, were assessed for a panel of representative strains of the genera and and . These data, together with the phylogenetic analyses, support the reclassification of as a separate species within the genus , comb. nov. (type strain B316=ATCC 51982=DSM 14932).

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Keyword(s): VFA, volatile fatty acid
© SGM
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2008-09-01
2024-04-19
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Reclassification of Clostridium proteoclasticum as Butyrivibrio proteoclasticus comb. nov., a butyrate-producing ruminal bacterium
Int J Syst Evol Microbiol 58, 2041 (2008); https://doi.org/10.1099/ijs.0.65845-0
/content/journal/ijsem/10.1099/ijs.0.65845-0
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Multiple sequence alignment of partial 16S rRNA genes from strains of the genera , , , and generated in CLUSTAL W. [ PDF] 136 KB

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