RT Journal Article SR Electronic(1) A1 Szoboszlay, Sándor A1 Atzél, Béla A1 Kukolya, József A1 Tóth, Erika M. A1 Márialigeti, Károly A1 Schumann, Peter A1 Kriszt, BalázsYR 2008 T1 Chryseobacterium hungaricum sp. nov., isolated from hydrocarbon-contaminated soil JF International Journal of Systematic and Evolutionary Microbiology, VO 58 IS 12 SP 2748 OP 2754 DO https://doi.org/10.1099/ijs.0.65847-0 PB Microbiology Society, SN 1466-5034, AB The taxonomic position of a strain isolated from kerosene-contaminated soil in Hungary and formerly misidentified as Brevundimonas vesicularis was examined using a polyphasic approach. The isolate, designated CHB-20pT, could be clearly assigned to the genus Chryseobacterium (family Flavobacteriaceae) on the basis of 16S rRNA gene sequence similarity. Strain CHB-20pT, a moderate oil degrader, was a Gram-negative, aerobic, mesophilic microbe with a temperature optimum of 28–30 °C. Predominant fatty acids were iso-C15 : 0, summed feature 3 (comprising C16 : 1 ω7c and/or iso-C15 : 0 2-OH) and iso-C17 : 0 3-OH. Menaquinone-6 (MK-6) was the predominant respiratory quinone; MK-5 was present as a minor component. The almost complete 16S rRNA gene sequence of strain CHB-20pT shared 94–97 % similarity with sequences of the type strains of species of the genus Chryseobacterium. DNA–DNA relatedness between strain CHB-20pT and its closest relative, Chryseobacterium caeni, was lower than 46 %. Moreover, several diagnostic phenotypic properties distinguished strain CHB-20pT from C. caeni. On the basis of biochemical, chemotaxonomic and genotypic data, isolate CHB-20pT represents a novel species within the genus Chryseobacterium, Chryseobacterium hungaricum sp. nov.; the type strain is CHB-20pT (=NCAIM B2269T=DSM 19684T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.65847-0