Crassaminicella profunda gen. nov., sp. nov., an anaerobic marine bacterium isolated from deep-sea sediments
Lakhal, Raja and Pradel, Nathalie and Postec, Anne and Ollivier, Bernard and Cayol, Jean-Luc and Godfroy, Anne and Fardeau, Marie-Laure and Galés, Grégoire,, 65, 3097-3102 (2015),
doi = https://doi.org/10.1099/ijsem.0.000386,
publicationName = Microbiology Society,
issn = 1466-5026,
abstract= A novel, anaerobic, chemo-organotrophic bacterium, designated strain Ra1766HT, was isolated from sediments of the Guaymas basin (Gulf of California, Mexico) taken from a depth of 2002 m. Cells were thin, motile, Gram-stain-positive, flexible rods forming terminal endospores. Strain Ra1766HT grew at temperatures of 25–45 °C (optimum 30 °C), pH 6.7–8.1 (optimum 7.5) and in a salinity of 5–60 g l− 1 NaCl (optimum 30 g l− 1). It was an obligate heterotrophic bacterium fermenting carbohydrates (glucose and mannose) and organic acids (pyruvate and succinate). Casamino acids and amino acids (glutamate, aspartate and glycine) were also fermented. The main end products from glucose fermentation were acetate, butyrate, ethanol, H2 and CO2. Sulfate, sulfite, thiosulfate, elemental sulfur, fumarate, nitrate, nitrite and Fe(III) were not used as terminal electron acceptors. The predominant cellular fatty acids were C14  : 0, C16 : 1ω7, C16 : 1ω7 DMA and C16 : 0. The main polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phospholipids. The G+C content of the genomic DNA was 33.7 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Ra1766HT was affiliated to cluster XI of the order Clostridiales, phylum Firmicutes. The closest phylogenetic relative of Ra1766HT was Geosporobacter subterraneus (94.2 % 16S rRNA gene sequence similarity). On the basis of phylogenetic inference and phenotypic properties, strain Ra1766HT ( = DSM 27501T = JCM 19377T) is proposed to be the type strain of a novel species of a novel genus, named Crassaminicella profunda.,
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