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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_10

sp. nov., an antibiotic-producing bacterium isolated from soil Free

Abstract

Strain T13, isolated from forest soil in Jeollabuk-do, South Korea, exhibited antibiotic production on yeast extract-malt extract-glucose (YMG) medium containing magnesium chloride as a trace mineral, and inhibited the growth of , , , , , , and . Growth occurred at 15–45 °C, pH 4–11 and in the presence of up to 2 % (w/v) NaCl. Biochemical analyses indicated that the predominant menaquinones produced by this strain were MK-9(H) and MK-9(H); small amounts of MK-10(H) and MK-10(H) were also detected. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, and the cell-wall peptidoglycan contained -diaminopimelic acid, glutamic acid, alanine and glycine. Whole-cell hydrolysates contained glucose, galactose, ribose and rhamnose. The fatty-acid profile of strain T13 was made up predominantly of iso- and anteiso-branched fatty acids. Genetic analyses demonstrated that strain T13 is closely related to JR-43 (98.29 % 16S rRNA gene sequence similarity), JR-19 (97.99 %), JR-41 (97.86 %), LMG 20096 (97.84 %), JL-22 (97.79 %) and Z4 (97.56 %), and DNA–DNA hybridization yielded relatedness values of 35.27–43.42 % when T13 was compared to related strains. The results of morphological, chemotaxonomic, phylogenetic and phenotypic analyses confirm that this strain represents a novel species of the genus , for which the name Streptomyces sp. nov. is proposed. The type strain is T13 ( = KEMB 9005-210 = KACC 18227 = NBRC 110901).

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Funding
This study was supported by the:
  • Ministry of Agriculture, Food, and Rural Affairs (Award 312027-3)
  • Ministry of Education, Science, and Technology (Award 2011-0010144)
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2015-10-01
2024-04-19
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Streptomyces olivicoloratus sp. nov., an antibiotic-producing bacterium isolated from soil
Int J Syst Evol Microbiol 65, 3262 (2015); https://doi.org/10.1099/ijsem.0.000404
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