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Volume 65, Issue Pt_10

sp. nov. and sp. nov., actinomycetes isolated from marine sediment of the South China Sea Free

Abstract

Two actinomycete strains, designated 10A08A and 10A08B, were isolated from marine sediment samples of the South China Sea and their taxonomic positions were determined by a polyphasic approach. The two Gram-stain-positive, aerobic strains produced branched substrate mycelium and aerial hyphae, and no diffusible pigment was produced in the media tested. At maturity, spore chains were formed on aerial hyphae and all mycelium fragmented with age. Whole-cell hydrolysates of both strains contained -diaminopimelic acid and no diagnostic sugars. Their predominant menaquinones (>10 %) were MK-9(H), MK-9(H) and MK-10(H) for strain 10A08A and MK-9(H), MK-9(H), MK-10(H) and MK-10(H) for strain 10A08B. The polar lipids detected from the two strains were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and unknown phosphoglycolipids and phospholipids. The major fatty acids (>10 %) of both strains were iso-C and summed feature 4 (iso-C I and/or anteiso-C B). The genomic DNA G+C contents of strains 10A08A and 10A08B were 70.9 and 71.6 mol%, respectively. On the basis of 16S rRNA gene sequence similarities, the two strains were shown to be most closely related to species of the genus . DNA–DNA hybridization relatedness values of < 70 % between these two isolates and their closest neighbour, YIM 90022, and between the two strains supported the conclusion that they represent two novel species. Based on phylogenetic analysis and phenotypic and genotypic data, it is concluded that the two isolates belong to the genus , and the names sp. nov. (type strain 10A08A = DSM 45931 = BCRC 16951) and sp. nov. (type strain 10A08B = CGMCC 47227 = BCRC 16952) are proposed.

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Funding
This study was supported by the:
  • National Basic Research Program of China
  • 863 Program
  • National Natural Science Foundation of China
© 2015 IUMS
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2015-10-01
2024-04-26
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References

  1. Athalye M., Goodfellow M., Lacey J., White R.P. ( 1985;). Numerical classification of Actinomadura and Nocardiopsis . Int J Syst Bacteriol 35 86–98 [View Article].
     [Google Scholar]
  2. Bernatchez H., Lebreux E. ( 1991;). Nocardiopsis dassonvillei recovered from a lung biopsy and a possible cause of extrinsic allergic alveolitis. Clin Microbiol Newsl 13 174–175 [View Article].
     [Google Scholar]
  3. Chen Y.G., Cui X.L., Kroppenstedt R.M., Stackebrandt E., Wen M.L., Xu L.H., Jiang C.L. ( 2008;). Nocardiopsis quinghaiensis sp. nov., isolated from saline soil in China. Int J Syst Evol Microbiol 58 699–705 [View Article] [PubMed].
     [Google Scholar]
  4. Chen Y.G., Wang Y.X., Zhang Y.Q., Tang S.K., Liu Z.X., Xiao H.D., Xu L.H., Cui X.L., Li W.J. ( 2009;). Nocardiopsis litoralis sp. nov., a halophilic marine actinomycete isolated from a sea anemone. Int J Syst Evol Microbiol 59 2708–2713 [View Article] [PubMed].
     [Google Scholar]
  5. Chen Y.G., Zhang Y.Q., Tang S.K., Liu Z.X., Xu L.H., Zhang L.X., Li W.J. ( 2010;). Nocardiopsis terrae sp. nov., a halophilic actinomycete isolated from saline soil. Antonie van Leeuwenhoek 98 31–38 [View Article] [PubMed].
     [Google Scholar]
  6. Chun J., Bae K.S., Moon E.Y., Jung S.O., Lee H.K., Kim S.J. ( 2000;). Nocardiopsis kunsanensis sp. nov., a moderately halophilic actinomycete isolated from a saltern. Int J Syst Evol Microbiol 50 1909–1913. [PubMed]. [CrossRef]
    [Google Scholar]
  7. Collins M.D., Jones D. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Bacteriol 48 459–470 [View Article].
     [Google Scholar]
  8. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221–230 [View Article] [PubMed].
     [Google Scholar]
  9. Ezaki T., Hashimoto Y., Takeuchi N., Yamamoto H., Liu S.-L., Miura H., Matsui K., Yabuuchi E. ( 1988;). Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. J Clin Microbiol 26 1708–1713. [PubMed].
     [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224–229 [View Article].
     [Google Scholar]
  11. Fang C., Zhang J., Pang H., Li Y., Xin Y., Zhang Y. ( 2011;). Nocardiopsis flavescens sp. nov., an actinomycete isolated from marine sediment. Int J Syst Evol Microbiol 61 2640–2645 [PubMed]. [CrossRef]
    [Google Scholar]
  12. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376 [View Article] [PubMed].
     [Google Scholar]
  13. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791 [View Article].
     [Google Scholar]
  14. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406–416 [View Article].
     [Google Scholar]
  15. Goodfellow M. ( 1971;). Numerical taxonomy of some nocardioform bacteria. J Gen Microbiol 69 33–80 [View Article] [PubMed].
     [Google Scholar]
  16. Hamedi J., Mohammadipanah F., von Jan M., Pötter G., Schumann P., Spröer C., Klenk H.P., Kroppenstedt R.M. ( 2010;). Nocardiopsis sinuspersici sp. nov., isolated from sandy rhizospheric soil. Int J Syst Evol Microbiol 60 2346–2352 [View Article] [PubMed].
     [Google Scholar]
  17. Hamedi J., Mohammadipanah F., Pötter G., Spröer C., Schumann P., Göker M., Klenk H.-P. ( 2011;). Nocardiopsis arvandica sp. nov., isolated from sandy soil. Int J Syst Evol Microbiol 61 1189–1194. [PubMed]. [CrossRef]
    [Google Scholar]
  18. Kämpfer P., Busse H.J., Rainey F.A. ( 2002;). Nocardiopsis compostus sp. nov., from the atmosphere of a composting facility. Int J Syst Evol Microbiol 52 621–627. [PubMed]. [CrossRef]
    [Google Scholar]
  19. Kawato N., Shinobu R. ( 1959;). On Streptomyces herbaricolor sp. nov. Supplement: a simple technique for the microscopic observation. Mem Osaka Univ Lib Arts Educ B Nat Sci 8 114–119.
     [Google Scholar]
  20. Kelly K.L. ( 1964). Inter-Society Color Council – National Bureau of Standards Color-Name Charts Illustrated with Centroid Colors Washington, DC: US Government Printing Office;.
     [Google Scholar]
  21. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  22. Kroppenstedt R.M., Evtushenko L.I. ( 2002;). The family Nocardiopsaceae . . In The Prokaryotes vol. 3 , 3rd edn.., pp. 754–795. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K.-H., Stackebrandt E. New York: Springer;.
     [Google Scholar]
  23. Lechevalier M.P., De Bièvre C., Lechevalier H.A. ( 1977;). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5 249–260 [View Article].
     [Google Scholar]
  24. Li M.G., Li W.J., Xu P., Cui X.L., Xu L.H., Jiang C.L. ( 2003;). Nocardiopsis xinjiangensis sp. nov., a halophilic actinomycete isolated from a saline soil sample in China. Int J Syst Evol Microbiol 53 317–321 [View Article] [PubMed].
     [Google Scholar]
  25. Li W.J., Park D.J., Tang S.K., Wang D., Lee J.C., Xu L.H., Kim C.J., Jiang C.L. ( 2004;). Nocardiopsis salina sp. nov., a novel halophilic actinomycete isolated from saline soil in China. Int J Syst Evol Microbiol 54 1805–1809 [View Article] [PubMed].
     [Google Scholar]
  26. Li W.-J., Kroppenstedt R.M., Wang D., Tang S.-K., Lee J.-C., Park D.-J., Kim C.-J., Xu L.-H., Jiang C.-L. ( 2006;). Five novel species of the genus Nocardiopsis isolated from hypersaline soils and emended description of Nocardiopsis salina Li et al. 2004. Int J Syst Evol Microbiol 56 1089–1096 [View Article] [PubMed].
     [Google Scholar]
  27. Li W.-J., Xu P., Schumann P., Zhang Y.-Q., Pukall R., Xu L.-H., Stackebrandt E., Jiang C.-L. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . Int J Syst Evol Microbiol 57 1424–1428 [View Article] [PubMed].
     [Google Scholar]
  28. Li J., Yang J., Zhu W.Y., He J., Tian X.P., Xie Q., Zhang S., Li W.J. ( 2012;). Nocardiopsis coralliicola sp. nov., isolated from the gorgonian coral, Menella praelonga . Int J Syst Evol Microbiol 62 1653–1658 [View Article] [PubMed].
     [Google Scholar]
  29. Marmur J. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3 208–218 [View Article].
     [Google Scholar]
  30. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159–167 [View Article].
     [Google Scholar]
  31. Meyer J. ( 1976;). Nocardiopsis, a new genus of the order Actinomycetales . Int J Syst Bacteriol 26 487–493 [View Article].
     [Google Scholar]
  32. Minnikin D.E., Collins M.D., Goodfellow M. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas Oerskovia and related taxa. J Appl Bacteriol 47 87–95 [View Article].
     [Google Scholar]
  33. Peltola J.S.P., Andersson M.A., Kämpfer P., Auling G., Kroppenstedt R.M., Busse H.J., Salkinoja-Salonen M.S., Rainey F.A. ( 2001;). Isolation of toxigenic Nocardiopsis strains from indoor environments and description of two new Nocardiopsis species, N. exhalans sp. nov. and N. umidischolae sp. nov.. Appl Environ Microbiol 67 4293–4304 [View Article] [PubMed].
     [Google Scholar]
  34. Pridham T.G., Gottlieb D. ( 1948;). The utilization of carbon compounds by some Actinomycetales as an aid for species determination. J Bacteriol 56 107–114. [PubMed].
     [Google Scholar]
  35. Qin S., Li J., Chen H.H., Zhao G.Z., Zhu W.Y., Jiang C.L., Xu L.H., Li W.J. ( 2009;). Isolation, diversity, and antimicrobial activity of rare actinobacteria from medicinal plants of tropical rain forests in Xishuangbanna, China. Appl Environ Microbiol 75 6176–6186 [View Article] [PubMed].
     [Google Scholar]
  36. Sabry S.A., Ghanem N.B., Abu-Ella G.A., Schumann P., Stackebrandt E., Kroppenstedt R.M. ( 2004;). Nocardiopsis aegyptia sp. nov., isolated from marine sediment. Int J Syst Evol Microbiol 54 453–456 [View Article] [PubMed].
     [Google Scholar]
  37. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425. [PubMed].
     [Google Scholar]
  38. Sasser M. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20 16.
     [Google Scholar]
  39. Shirling E.B., Gottlieb D. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16 313–340 [View Article].
     [Google Scholar]
  40. Stackebrandt E., Ebers J. ( 2006;). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33 152–155.
     [Google Scholar]
  41. Staneck J.L., Roberts G.D. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28 226–231 [PubMed].
     [Google Scholar]
  42. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. ( 1983;). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 54 31–36 [View Article].
     [Google Scholar]
  43. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 2731–2739 [View Article] [PubMed].
     [Google Scholar]
  44. Tang S.-K., Wang Y., Chen Y., Lou K., Cao L.-L., Xu L.-H., Li W.-J. ( 2009;). Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella . Int J Syst Evol Microbiol 59 2025–2032 [View Article] [PubMed].
     [Google Scholar]
  45. Thompson J.D., Gibson T.J., Plewniak F., Jeanmougin F., Higgins D.G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 4876–4882 [View Article] [PubMed].
     [Google Scholar]
  46. Vasanthi V., Hoti S.L. ( 1992;). Microbial flora in gut of Culex quinquefasciatus breeding in cess pits. Southeast Asian J Trop Med Public Health 23 312–317. [PubMed].
     [Google Scholar]
  47. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463–464 [View Article].
     [Google Scholar]
  48. Xu P., Li W.J., Tang S.K., Zhang Y.Q., Chen G.Z., Chen H.H., Xu L.H., Jiang C.L. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 55 1149–1153 [View Article] [PubMed].
     [Google Scholar]
  49. Yassin A.F., Rainey F.A., Burghardt J., Gierth D., Ungerechts J., Lux I., Seifert P., Bal C., Schaal K.P. ( 1997;). Description of Nocardiopsis synnemataformans sp. nov., elevation of Nocardiopsis alba subsp. prasina to Nocardiopsis prasina comb. nov., and designation of Nocardiopsis antarctica and Nocardiopsis alborubida as later subjective synonyms of Nocardiopsis dassonvillei . Int J Syst Bacteriol 47 983–988 [View Article] [PubMed].
     [Google Scholar]
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Nocardiopsis oceani sp. nov. and Nocardiopsis nanhaiensis sp. nov., actinomycetes isolated from marine sediment of the South China Sea
Int J Syst Evol Microbiol 65, 3384 (2015); https://doi.org/10.1099/ijsem.0.000425
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