1887

Abstract

A Gram-reaction-negative, non-motile, facultatively aerobic bacterium, designated strain M1, was isolated from a subterrestrial sediment sample of Qiangtang Basin in Qinghai-Tibetan plateau, China. The strain formed rough yellow colonies on R2A plates. Cells were oval or short rod-shaped, catalase-positive and oxidase-negative. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the isolate belonged to the family and showed 96.2–96.4 % 16S rRNA gene sequence similarities to its closest relatives. Chemotaxonomic analysis revealed ubiquinone-10 (Q10) as the dominant respiratory quinone of strain M1 and Cω6 (44.2 %) and Cω7 (13.7 %) as the major fatty acids. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, sphingoglycolipid, three unidentified glycolipids, one unidentified phosphoglycolipid and one unidentified lipid. The DNA G+C content of strain M1 was 73.7 mol%. On the basis of phenotypic, phylogenetic and genotypic data presented in this study, strain M1 represents a novel species of a new genus in the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is M1 ( = CGMCC 1.12928 = JCM 30182).

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2015-10-01
2024-03-29
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References

  1. Barrow G. I., Feltham R. K. A. (editors), ( 1993). Cowan and Steel's Manual for the Identification of Medical Bacteria , 3rd edn. Cambridge: Cambridge University Press; [View Article].
    [Google Scholar]
  2. Bernardet J.F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52 10491070 [View Article] [PubMed].
    [Google Scholar]
  3. da Costa M.S., Albuquerque L., Nobre M.F., Wait R. ( 2011;). The extraction and identification of respiratory lipoquinones of prokaryotes and their use in taxonomy. Methods Microbiol 38 197206 [View Article].
    [Google Scholar]
  4. Fan Z.Y., Xiao Y.P., Hui W., Tian G.R., Lee J.S., Lee K.C., Quan Z.X. ( 2011;). Altererythrobacter dongtanensis sp. nov., isolated from a tidal flat. Int J Syst Evol Microbiol 61 20352039 [View Article] [PubMed].
    [Google Scholar]
  5. Fautz E., Reichenbach H. ( 1980;). A simple test for flexirubin-type pigments. FEMS Microbiol Lett 8 8791 [View Article].
    [Google Scholar]
  6. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791. [CrossRef]
    [Google Scholar]
  7. Fitch W.M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  8. Fuerst J.A., Hawkins J.A., Holmes A., Sly L.I., Moore C.J., Stackebrandt E. ( 1993;). Porphyrobacter neustonensis gen. nov., sp. nov., an aerobic bacteriochlorophyll-synthesizing budding bacterium from fresh water. Int J Syst Bacteriol 43 125134 [View Article] [PubMed].
    [Google Scholar]
  9. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. (editors), ( 1994). Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology;.
    [Google Scholar]
  10. Han L., Wu S.J., Qin C.Y., Zhu Y.H., Lu Z.Q., Xie B., Lv J. ( 2014;). Hymenobacter qilianensis sp. nov., isolated from a subsurface sandstone sediment in the permafrost region of Qilian Mountains, China and emended description of the genus Hymenobacter . Antonie van Leeuwenhoek 105 971978 [View Article] [PubMed].
    [Google Scholar]
  11. Huang F., Zhang Y., Zhu Y., Wang P., Lu J., Lv J. ( 2014;). Flavobacterium qiangtangensis sp. nov., isolated from Qiangtang basin in Qinghai-Tibetan Plateau, China. Curr Microbiol 69 234239 [View Article] [PubMed].
    [Google Scholar]
  12. Huang Y., Zeng Y., Feng H., Wu Y., Xu X. ( 2015;). Croceicoccus naphthovorans sp. nov., a polycyclic aromatic hydrocarbons-degrading and acylhomoserine-lactone-producing bacterium isolated from marine biofilm, and emended description of the genus Croceicoccus . Int J Syst Evol Microbiol 65 15311536 . http://dx.doi.org/10.1099/ijs.0.000132 . [CrossRef]
    [Google Scholar]
  13. Jeong S.H., Jin H.M., Lee H.J., Jeon C.O. ( 2013;). Altererythrobacter gangjinensis sp. nov., a marine bacterium isolated from a tidal flat. Int J Syst Evol Microbiol 63 971976 [View Article] [PubMed].
    [Google Scholar]
  14. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed].
    [Google Scholar]
  15. Kimura M. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16 111120 [View Article] [PubMed].
    [Google Scholar]
  16. Kishino H., Hasegawa M. ( 1989;). Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in hominoidea . J Mol Evol 29 170179 [View Article] [PubMed].
    [Google Scholar]
  17. Kwon K.K., Woo J.-H., Yang S.-H., Kang J.-H., Kang S.G., Kim S.-J., Sato T., Kato C. ( 2007;). Altererythrobacter epoxidivorans gen. nov., sp. nov., an epoxide hydrolase-active, mesophilic marine bacterium isolated from cold-seep sediment, and reclassification of Erythrobacter luteolus Yoon et al. 2005 as Altererythrobacter luteolus comb. nov.. Int J Syst Evol Microbiol 57 22072211 [View Article] [PubMed].
    [Google Scholar]
  18. Lee K-B., Liu C.-T., Anzai Y., Kim H., Aono T., Oyaizu H. ( 2005;). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.. Int J Syst Evol Microbiol 55 19071919 [View Article] [PubMed].
    [Google Scholar]
  19. Lei X., Li Y., Chen Z., Zheng W., Lai Q., Zhang H., Guan C., Cai G., Yang X., other authors. ( 2014;). Altererythrobacter xiamenensis sp. nov., an algicidal bacterium isolated from red tide seawater. Int J Syst Evol Microbiol 64 631637 [View Article] [PubMed].
    [Google Scholar]
  20. Mesbah M., Whitman W.B. ( 1989;). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr A 479 297306 [View Article] [PubMed].
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W.B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159167 [View Article].
    [Google Scholar]
  22. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  23. Nogi Y., Yoshizumi M., Miyazaki M. ( 2014;). Thalassospira povalilytica sp. nov., a polyvinyl-alcohol-degrading marine bacterium. Int J Syst Evol Microbiol 64 11491153 [View Article] [PubMed].
    [Google Scholar]
  24. Rainey F.A., Silva J., Nobre M.F., Silva M.T., da Costa M.S. ( 2003;). Porphyrobacter cryptus sp. nov., a novel slightly thermophilic, aerobic, bacteriochlorophyll a-containing species. Int J Syst Evol Microbiol 53 3541 [View Article] [PubMed].
    [Google Scholar]
  25. Rzhetsky A., Nei M. ( 1993;). Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10 10731095 [PubMed].
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425. [PubMed].
    [Google Scholar]
  27. Shiba T., Simidu U. ( 1982;). Erythrobacter longus gen. nov., sp. nov., an aerobic bacterium which contains bacteriochlorophyll a . Int J Syst Bacteriol 32 211217 [View Article].
    [Google Scholar]
  28. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch.V. ( 2013;). Erythrobacter odishensis sp. nov. and Pontibacter odishensis sp. nov. isolated from dry soil of a solar saltern. Int J Syst Evol Microbiol 63 45244532 [View Article] [PubMed].
    [Google Scholar]
  29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed].
    [Google Scholar]
  30. Tan X., Zhang R.G., Meng T.Y., Liang H.Z., Lv J. ( 2014;). Taibaiella chishuiensis sp. nov., isolated from freshwater. Int J Syst Evol Microbiol 64 17951801 [View Article] [PubMed].
    [Google Scholar]
  31. Thompson J.D., Higgins D.G., Gibson T.J. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 46734680 [View Article] [PubMed].
    [Google Scholar]
  32. Wu Y.H., Xu L., Meng F.X., Zhang D.S., Wang C.S., Oren A., Xu X.W. ( 2014;). Altererythrobacter atlanticus sp. nov., isolated from deep-sea sediment. Int J Syst Evol Microbiol 64 116121 [View Article] [PubMed].
    [Google Scholar]
  33. Xu X.-W., Wu Y.-H., Wang C.-S., Wang X.-G., Oren A., Wu M. ( 2009;). Croceicoccus marinus gen. nov., sp. nov., a yellow-pigmented bacterium from deep-sea sediment, and emended description of the family Erythrobacteraceae . Int J Syst Evol Microbiol 59 22472253 [View Article] [PubMed].
    [Google Scholar]
  34. Xu M., Xin Y., Yu Y., Zhang J., Zhou Y., Liu H., Tian J., Li Y. ( 2010;). Erythrobacter nanhaisediminis sp. nov., isolated from marine sediment of the South China Sea. Int J Syst Evol Microbiol 60 22152220 [View Article] [PubMed].
    [Google Scholar]
  35. Xue X., Zhang K., Cai F., Dai J., Wang Y., Rahman E., Peng F., Fang C. ( 2012;). Altererythrobacter xinjiangensis sp. nov., isolated from desert sand, and emended description of the genus Altererythrobacter . Int J Syst Evol Microbiol 62 2832 [View Article] [PubMed].
    [Google Scholar]
  36. Yoon J.-H., Kang K.H., Yeo S.-H., Oh T.-K. ( 2005;). Erythrobacter luteolus sp. nov., isolated from a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 55 11671170 [View Article] [PubMed].
    [Google Scholar]
  37. Yoon J.H., Kang S.J., Lee M.H., Oh H.W., Oh T.K. ( 2006;). Porphyrobacter dokdonensis sp. nov., isolated from sea water. Int J Syst Evol Microbiol 56 10791083 [View Article] [PubMed].
    [Google Scholar]
  38. Yurkov V., Stackebrandt E., Holmes A., Fuerst J.A., Hugenholtz P., Golecki J., Gad'on N., Gorlenko V.M., Kompantseva E.I., Drews G. ( 1994;). Phylogenetic positions of novel aerobic, bacteriochlorophyll a-containing bacteria and description of Roseococcus thiosulfatophilus gen. nov., sp. nov., Erythromicrobium ramosum gen. nov., sp. nov., and Erythrobacter litoralis sp. nov.. Int J Syst Bacteriol 44 427434 [View Article] [PubMed].
    [Google Scholar]
  39. Zhang R.G., Tan X., Zhao X.M., Deng J., Lv J. ( 2014;). Moheibacter sediminis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from sediment, and emended descriptions of Empedobacter brevis, Wautersiella falsenii and Weeksella virosa . Int J Syst Evol Microbiol 64 14811487 [View Article] [PubMed].
    [Google Scholar]
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