Microbacterium nanhaiense sp. nov., an actinobacterium isolated from sea sediment Yan, Lien and Wang, Jingjing and Chen, Zhirong and Guan, Yingying and Li, Jing,, 65, 3697-3702 (2015), doi = https://doi.org/10.1099/ijsem.0.000480, publicationName = Microbiology Society, issn = 1466-5026, abstract= A Gram-staining-positive, heterotrophic, anaerobic, non-spore-forming, non-motile, rod-shaped strain, OAct400T, belonging to the genus Microbacterium was isolated from a sediment collected from a depth of 2093 m in the South China Sea, China. The strain was identified using a polyphasic taxonomic approach. The strain grew well on yeast extract/malt extract agar (ISP 2) and nutrient agar media, and formed no aerial mycelium and no diffusible pigments on any media tested. The strain grew in the presence of 0–8 % (w/v) NaCl (optimum, 2–4 %), at pH 5.0–10.0 (optimum, pH 7.0) and at 4–37 °C (optimum, 28 °C). Strain OAct400T contained ornithine as the diagnostic diamino acid. The whole-cell sugars were dominated by glucose and galactose. The predominant menaquinones were MK-11 (51 %) and MK-10 (24 %). The major phospholipids were phosphatidylglycerol and diphosphatidylglycerol. The major fatty acids were anteiso-C15 : 0 (59.35 %), iso-C16 : 0 (17.89 %) and anteiso-C17 : 0 (16.09 %). DNA–DNA relatedness with Microbacterium amylolyticum DSM 24221T and Microbacterium gubbeenense CIP 107184T, the nearest phylogenetic relatives (97.73 and 97.44 % 16S rRNA gene sequence similarity, respectively) was 31.3 ± 2.1 and 28.7 ± 1.2 %, respectively. On the basis of phenotypic, phylogenetic and genotypic data, a novel species, Microbacterium nanhaiense sp. nov., is proposed. The type strain is OAct400T ( = CGMCC 4.7181T = DSM 26811T = KCTC 29185T)., language=, type=