@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000555, author = "Kämpfer, Peter and Busse, Hans-Jürgen and McInroy, John A. and Glaeser, Stefanie P.", title = "Bacillus gossypii sp. nov., isolated from the stem of Gossypium hirsutum", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2015", volume = "65", number = "Pt_11", pages = "4163-4168", doi = "https://doi.org/10.1099/ijsem.0.000555", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000555", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A Gram-stain-positive, facultatively anaerobic, endospore-forming organism, isolated from the stem of Gossypium hirsutum, was studied to determine its taxonomic position. On the basis of 16S rRNA gene sequence similarity comparisons, strain JM-267T was grouped in the genus Bacillus, related most closely to the type strains of Bacillus simplex and Bacillus huizhouensis (both 97.8 %), Bacillus muralis (97.7 %), Bacillus butanolivorans and Bacillus psychrosaccharolyticus (both 97.3 %). 16S rRNA gene sequence similarity to the sequences of the type strains of other Bacillus species was < 97.0 %. The fatty acid profile supported the grouping of the strain to the genus Bacillus. As major fatty acids, anteiso-C15 : 0, iso-C15 : 0, iso-C14 : 0 and iso-C16 : 0 were detected. The polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major quinone was menaquinone 7 (MK-7). DNA–DNA hybridizations with B. simplex DSM 1321T, B. huizhouensis GSS03T, B. muralis LMG 20238T, B. butanolivorans LMG 23974T and B. psychrosaccharolyticus DSM 6T resulted in values clearly below 70 %. In addition, physiological and biochemical test results allowed the clear phenotypic differentiation of strain JM-267T from the most closely related species. Hence, strain JM-267T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus gossypii sp. nov. is proposed. The type strain is JM-267T ( = DSM 100034T = LMG 28742T).", }