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Volume 65, Issue Pt_11

sp. nov., isolated from soil Free

Abstract

A novel filamentous bacterial strain, A-T 5190, which developed irregular sporangia at the end of sporangiophores on substrate mycelia, was isolated from dry evergreen forest soil collected in Thailand. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 5190 belonged to the genus and was related most closely to NBRC 14916 (98.88 % similarity) and NBRC 13941 (98.54 %). DNA–DNA relatedness values between strain A-T 5190 and its closest relatives were below 70 %. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, galactose and xylose. The predominant menaquinone was MK-9(H). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were unsaturated fatty acid C and branched fatty acids iso-C, iso-C and anteiso-C. The G+C content of the genomic DNA was 71.9 mol%. Evidence from phenotypic, chemotaxonomic and genotypic studies indicate that strain A-T 5190 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A-T 5190 ( = BCC 41582 = NBRC 109644).

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2015-11-01
2024-04-19
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References

  1. Arai T. ( 1975). Culture Media for Actinomycetes., Tokyo: The Society for Actinomycetes Japan;.
     [Google Scholar]
  2. Collins M.D., Pirouz T., Goodfellow M., Minnikin D.E. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100 221–230 [View Article] [PubMed].
     [Google Scholar]
  3. Couch J.N. ( 1950;). Actinoplanes, a new genus of the Actinomycetales . J Elisha Mitchell Sci Soc 66 87–92.
     [Google Scholar]
  4. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39 224–229 [View Article].
     [Google Scholar]
  5. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368–376 [View Article] [PubMed].
     [Google Scholar]
  6. Felsenstein J. ( 1983;). Parsimony in systematics: biological and statistical issues. Annu Rev Ecol Syst 14 313–333 [View Article].
     [Google Scholar]
  7. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783–791 [View Article].
     [Google Scholar]
  8. Goodfellow M., Stanton L.J., Simpson K.E., Minnikin D.E. ( 1990;). Numerical and chemical classification of Actinoplanes and some related actinomycetes. J Gen Microbiol 136 19–36 [View Article].
     [Google Scholar]
  9. Gordon R.E., Barnett D.A., Handerhan J.E., Pang C.H.-N. ( 1974;). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24 54–63 [View Article].
     [Google Scholar]
  10. Greenwood J.R., Pickett M.J. ( 1979;). Salient features of Haemophilus vaginalis . J Clin Microbiol 9 200–204 [PubMed].
     [Google Scholar]
  11. Itoh T., Kudo T., Parenti F., Seino A. ( 1989;). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39 168–173 [View Article].
     [Google Scholar]
  12. Kämpfer P., Kroppenstedt R.M. ( 1996;). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42 989–1005 [View Article].
     [Google Scholar]
  13. Katsura K., Kawasaki H., Potacharoen W., Saono S., Seki T., Yamada Y., Uchimura T., Komagata K. ( 2001;). Asaia siamensis sp. nov., an acetic acid bacterium in the alpha-proteobacteria. Int J Syst Evol Microbiol 51 559–563 [View Article] [PubMed].
     [Google Scholar]
  14. Kawasaki H., Hoshino Y., Hirata A., Yamasato K. ( 1993;). Is intracytoplasmic membrane structure a generic criterion? It does not coincide with phylogenetic interrelationships among phototrophic purple nonsulfur bacteria. Arch Microbiol 160 358–362 [View Article] [PubMed].
     [Google Scholar]
  15. Kelly K.L. ( 1964). Inter-Society Color Council – National Bureau of Standard Color Name Charts Illustrated with Centroid Colors., Washington, DC: US Government Printing Office;.
     [Google Scholar]
  16. Kim O.S., Cho Y.J., Lee K., Yoon S.H., Kim M., Na H., Park S.C., Jeon Y.S., Lee J.H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716–721 [View Article] [PubMed].
     [Google Scholar]
  17. Komagata K., Suzuki K.I. ( 1987;). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19 161–207 [View Article].
     [Google Scholar]
  18. Lechevalier M.P., Lechevalier H. ( 1970;). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20 435–443 [View Article].
     [Google Scholar]
  19. Minnikin D.E., O'Donnell A.G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J.H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233–241 [View Article].
     [Google Scholar]
  20. Saito H., Miura K.I. ( 1963;). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72 619–629 [View Article] [PubMed].
     [Google Scholar]
  21. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406–425 [PubMed].
     [Google Scholar]
  22. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids., MIDI Technical Note 101 Newark, DE: MIDI Inc;.
     [Google Scholar]
  23. Sazak A., Sahin N., Camas M. ( 2012;). Actinoplanes abujensis sp. nov., isolated from Nigerian arid soil. Int J Syst Evol Microbiol 62 960–965 [View Article] [PubMed].
     [Google Scholar]
  24. Shirling E.B., Gottlieb D. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16 313–340 [View Article].
     [Google Scholar]
  25. Staneck J.L., Roberts G.D. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbio 28 226–231 [PubMed].
     [Google Scholar]
  26. Suriyachadkun C., Chunhametha S., Thawai C., Tamura T., Potacharoen W., Kirtikara K., Sanglier J.J. ( 2009;). Planotetraspora thailandica sp. nov., isolated from soil in Thailand. Int J Syst Evol Microbiol 59 992–997 [View Article] [PubMed].
     [Google Scholar]
  27. Suzuki S.I. ( 2001;). Establishment and use of gellan gum media for selective isolation and distribution survey of specific rare actinomycetes. Actinomycetologica 15 55–60 [View Article].
     [Google Scholar]
  28. Tamaoka J., Komagata K. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25 125–128. [CrossRef]
    [Google Scholar]
  29. Tamura T., Hatano K. ( 2001;). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanes minutisporangius Ruan et al. 1986 and ‘Actinoplanes aurantiacus’ to Cryptosporangium minutisporangium comb. nov. and Cryptosporangium aurantiacum sp. nov. Int J Syst Evol Microbiol 51 2119–2125 [View Article] [PubMed].
     [Google Scholar]
  30. Tamura T., Nakagaito Y., Nishii T., Hasegawa T., Stackebrandt E., Yokota A. ( 1994;). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44 193–203 [View Article] [PubMed].
     [Google Scholar]
  31. Thompson J.D., Higgins D.G., Gibson T.J. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22 4673–4680 [View Article] [PubMed].
     [Google Scholar]
  32. Uchida K., Aida K. ( 1984;). An improved method for the glycolate test for simple identification of the acyl type of bacterial cell walls. J Gen Appl Microbiol 30 131–134 [View Article].
     [Google Scholar]
  33. Verlander C.P. ( 1992;). Detection of horseradish peroxidase by colorimetry. . In Nonisotopic DNA Probe Techniques, pp. 185–201. Edited by Kricka L. J. New York: Academic Press; [View Article].
     [Google Scholar]
  34. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.E., other authors. ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37 463–464 [View Article].
     [Google Scholar]
  35. Williams S.T., Cross T. ( 1971;). Actinomycetes. Methods Microbiol 4 295–334 [View Article].
     [Google Scholar]
  36. Wu C., Lu X., Qin M., Wang Y., Ruan J. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16 (176), 178.
     [Google Scholar]
  37. Yamada Y., Katsura K., Kawasaki H., Widyastuti Y., Saono S., Seki T., Uchimura T., Komagata K. ( 2000;). Asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the alpha-Proteobacteria . Int J Syst Evol Microbiol 50 823–829 [View Article] [PubMed].
     [Google Scholar]
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Actinoplanes luteus sp. nov., isolated from soil
Int J Syst Evol Microbiol 65, 4227 (2015); https://doi.org/10.1099/ijsem.0.000562
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