1887

Abstract

gen. nov., sp. nov. was obtained from samples of sediment collected in a brackish lagoon of Maricá city, Rio de Janeiro state, Brazil. The morphological description was based on live observations, after protargol staining, and scanning electron microscopy. The novel species has a cylindrical body shape that is slightly contractile, 230–550 × 35–65 μm, a cytoplasm with many globular inclusions, one row of irregular cortical granules between each somatic kinety, approximately 40–62 somatic kineties, two globular macronuclei measuring 9–24 μm and one micronucleus of approximately 4–9 μm. A subapical oral cavity was approximately 20–80 × 9–25 μm, with an adoral zone on the left side of the buccal field, which was composed of 32–60 polykineties and a paroral at the right side that was composed of 40–57 short polykineties. The new genus is distinguished from other geleiids by a loop-shaped posterior end of the paroral ciliature, made up of two rows of short polykineties, and the oralization of the central superior kinety (K0i), forming a row of dikinetids that borders the adoral zone internally, followed by several rows of monokinetids. In the phylogenetic analyses, the novel species was recovered as the sister group of with full support values based on 18S rRNA gene sequences. This work also indicates some problems in the definitions of the Geleiidae and proposes a new diagnosis for this karyorelictid family.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000579
2015-12-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4297.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000579&mimeType=html&fmt=ahah

References

  1. Castresana J. ( 2000;). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17 540552 [View Article] [PubMed].
    [Google Scholar]
  2. Darriba D., Taboada G. L., Doallo R., Posada D. ( 2012;). jModelTest 2: more models, new heuristics and parallel computing. Nat Methods 9 772 [View Article] [PubMed].
    [Google Scholar]
  3. Dieckmann J. ( 1995;). An improved protargol impregnation for ciliates yielding reproducible results. Eur J Protistol 31 372382 [View Article].
    [Google Scholar]
  4. Dragesco J. ( 1999). Revision des Geléiides (Ciliophora, Karyorelictea) (Stapfia, vol. 66)., Linz: Botanische Arbeitsgemeinschaft am O.Ö. Landesmuseum Linz (in French);.
    [Google Scholar]
  5. Foissner W. ( 1991;). Basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa. Eur J Protistol 27 313330 [View Article] [PubMed].
    [Google Scholar]
  6. Foissner W. ( 1992;). Estimating the species richness of soil protozoa using the “non flooded Petri dish method”. . In Protocols in Protozoology, pp. B101102. Edited by Lee J. J., Soldo A. T. Lawrence, KS: Allen Press;.
    [Google Scholar]
  7. Foissner W. ( 1998;). The karyorelictids (Protozoa: Ciliophora), a unique and enigmatic assemblage of marine, interstitial ciliates: a review emphasizing ciliary patterns and evolution. . In Evolutionary Relationships among Protozoa, pp. 305325. Edited by Coombs G. H., Vickerman K., Sleig M. A., Warren A. London: Chapman & Hall;.
    [Google Scholar]
  8. Gao F., Katz L. A. ( 2014;). Phylogenomic analyses support the bifurcation of ciliates into two major clades that differ in properties of nuclear division. Mol Phylogenet Evol 70 240243 [View Article] [PubMed].
    [Google Scholar]
  9. Hausmann K., Hülsmann N. ( 1996). Protozoology , 2nd edn. New York: Thieme Medical Publishers;.
    [Google Scholar]
  10. Jankowski A. W. ( 2007;). Phylum Ciliophora Doflein, 1901. . In Protista, Part 2, Handbook on Zoology, pp. 415993. Edited by Alimov A. F. St. Petersburg: Russian Academy of Sciences, Zoological Institute;.
    [Google Scholar]
  11. Lynn D. H. ( 2008). The Ciliated Protozoa: Characterization, Classification, and Guide to the Literature , 3rd edn. Dordrecht: Springer;.
    [Google Scholar]
  12. Lynn D. H., Small E. B. ( 2002;). Phylum Ciliophora, Doflein, 1901. . In An Illustrated Guide to the Protozoa, pp. 371656. Edited by Lee J. J., Leedale G. F., Bradbury P. C. New York: Wiley-Blackwell;.
    [Google Scholar]
  13. Medlin L., Elwood H. J., Stickel S., Sogin M. L. ( 1988;). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71 491499 [View Article] [PubMed].
    [Google Scholar]
  14. Messing J. ( 1983;). New M13 vectors for cloning. Methods Enzymol 101 2078 [View Article] [PubMed].
    [Google Scholar]
  15. Nouzarède M. ( 1977;). Cytologie fonctionnelle et morphologie expérimentale de quelques protozoaires ciliés mésopsammiques géants de la famille des Geleidae (Kahl) (Bulletin de la Station Biologique d'Arcachon, N.S. 28). Arcachon, France: Société Scientifique d'Arcachon (in French);.
    [Google Scholar]
  16. Puytorac P. ( 1994;). Classe des Karyorelictea Corliss, 1974. . In Traité de Zoologie, pp. 2134. Edited by Grassé P. P. Paris: Masson (in French);.
    [Google Scholar]
  17. Ronquist F., Huelsenbeck J. P. ( 2003;). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19 15721574 [View Article] [PubMed].
    [Google Scholar]
  18. Silva-Neto I. D., da Silva Paiva T., Pedroso Dias R. J., Alexandre Campos C. J., Migotto A. E. ( 2012;). Redescription of Licnophora chattoni Villeneuve-Brachon, 1939 (Ciliophora, Spirotrichea), associated with Zyzzyzus warreni Calder, 1988 (Cnidaria, Hydrozoa). Eur J Protistol 48 4862 [View Article] [PubMed].
    [Google Scholar]
  19. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed].
    [Google Scholar]
  20. Wilm A., Higgins D. G., Notredame C. ( 2008;). R-Coffee: a method for multiple alignment of non-coding RNA. Nucleic Acids Res 36 e52 [View Article].
    [Google Scholar]
  21. Xu Y., Li J., Song W., Warren A. ( 2013;). Phylogeny and establishment of a new ciliate family, Wilbertomorphidae fam. nov. (Ciliophora, Karyorelictea), a highly specialized taxon represented by Wilbertomorpha colpoda gen. nov., spec. nov. J Eukaryot Microbiol 60 480489 [View Article] [PubMed].
    [Google Scholar]
  22. Xu Y., Pan H., Miao M., Hu X., Al-Farraj S. A., Al-Rasheid K. A. S., Song W. ( 2015;). Morphology and phylogeny of two species of Loxodes (Ciliophora, Karyorelictea), with description of a new subspecies, Loxodes striatus orientalis subsp. n. J Eukaryot Microbiol 62 206216 [View Article] [PubMed].
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000579
Loading
/content/journal/ijsem/10.1099/ijsem.0.000579
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error