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Volume 65, Issue Pt_12

sp. nov., isolated from Pu′er tea Free

Abstract

A novel Gram-reaction-positive, aerobic and non-spore-forming rod-shaped bacterial strain, YS17, was isolated from ripened Pu′er tea. Growth of the strain was observed at 15–50 °C (optimum 30–37 °C) and at pH 5.5–10.5 (optimum 6.0–9.5). Phylogenetic analysis of 16S rRNA gene sequences indicated that the strain represented a member of the genus . The strains most closely related to YS17 were DSM 8599, JCM 13518 and JCM 14732, with 16S rRNA gene sequence similarities of 96.8, 96.8 and 96.7 %, respectively. DNA–DNA hybridization of YS17 with the type strains of the most closely related species, DSM 8599, JCM 13518 and JCM 14732, yielded reassociation values of 10.9, 16.8 and 10.9 %, respectively. The diagnostic diamino acid of the cell wall peptidoglycan was -diaminopimelic acid. The predominant menaquinones were menaquinone MK-9(H) (76 %) and MK-8(H) (17 %). The major fatty acids were C, 10-methyl C and Cω9. The DNA G+C content of YS17 was 66 mol%. YS17 could be differentiated from recognized species of the genus on the basis of phenotypic characteristics, chemotaxonomic differences, phylogenetic analysis and DNA–DNA hybridization data. On the basis of evidence from the polyphasic analyses performed as part of this study a novel species, sp. nov., is proposed, with strain YS17 ( = CGMCC 1.12942 = JCM 30952) as the type strain.

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31200007)
  • Anhui Provincial Natural Science Foundation (Award 1308085MC31)
© 2015 IUMS

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sp. nov., isolated from Pu’er tea
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2015-12-01
2024-04-24
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Aeromicrobium camelliae sp. nov., isolated from Pu′er tea
Int J Syst Evol Microbiol 65, 4369 (2015); https://doi.org/10.1099/ijsem.0.000583
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