1887

Abstract

A novel, Gram-negative marine bacterium, S2753, was isolated from a mussel of the Solomon Sea, Solomon Islands. Analysis of the 16S rRNA gene sequence and whole genome sequence data placed strain S2753 in the genus with the closest relative being DSM 18316 (97.7 % 16S rRNA gene similarity). Strain S2753 was able to grow from 15 to 40 °C and in NaCl concentrations of 0.5 to 9 % (w/v). The predominant fatty acids were 16 : 1ω7/16 : 1ω6 (27.9 %), 16 : 0 (22.1 %) and 18 : 1ω7/8 : 1ω6c (21.4 %). The genomic DNA G+C mol content was 49.5 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic differences, strain S2753 is considered to represent a novel species of the genus . Furthermore, whole genome sequence analysis comparing S2753 and type-strains of closely related species of the genus also demonstrated that the strain is genomically distinct enough to be considered a novel species. The name is proposed and the type-strain is S2753( = LMG 28894 = DSM 100496).

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2015-12-01
2024-03-28
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References

  1. Auch A. F., von Jan M., Klenk H. -P., Göker M., Digital D. N. A- D. N. A. ( 2010a;). hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci 2 117134 [View Article] [PubMed] .
    [Google Scholar]
  2. Auch A. F., Klenk H. -P., Göker M. ( 2010b;). Standard operating procedure for calculating genome-to-genome distances based on high-scoring segment pairs. Stand Genomic Sci 2 142148 [View Article] [PubMed] .
    [Google Scholar]
  3. Beijerinck M. W. ( 1889;). Le Photobacterium luminosum, Bactérie lumineuse de la Mer du Nord. Arch Néerlandaises des Sci Exactes Nat 401427.
    [Google Scholar]
  4. Cowan S. T. ( 1974). Cowan and Steel's Manual for the Identification of Medical Bacteria , 2nd edn. Cambridge: Cambridge University Press;.
    [Google Scholar]
  5. Gabriel M. W., Matsui G. Y., Friedman R., Lovell C. R. ( 2014;). Optimization of multilocus sequence analysis for identification of species in the genus Vibrio . Appl Environ Microbiol 80 53595365 [View Article] [PubMed] .
    [Google Scholar]
  6. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. ( 2007;). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57 8191 [View Article] [PubMed] .
    [Google Scholar]
  7. Gram L., Melchiorsen J., Bruhn J. B. ( 2010;). Antibacterial activity of marine culturable bacteria collected from a global sampling of ocean surface waters and surface swabs of marine organisms. Mar Biotechnol (NY) 12 439451 [View Article] [PubMed] .
    [Google Scholar]
  8. Gregersen T. ( 1978;). Rapid method for distinction of gram-negative from gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5 123127 [View Article].
    [Google Scholar]
  9. Machado H., Gram L. ( 2015;). The fur gene as a new phylogenetic marker for Vibrionaceae species identification. Appl Environ Microbiol 81 27452752 [View Article] [PubMed] .
    [Google Scholar]
  10. Machado H., Månsson M., Gram L. ( 2014;). Draft genome sequence of Photobacterium halotolerans S2753, producer of bioactive secondary metabolites. Genome Announc 2 e0053514 [View Article] [PubMed] .
    [Google Scholar]
  11. Mansson M., Gram L., Larsen T. O. ( 2011a;). Production of bioactive secondary metabolites by marine vibrionaceae . Mar Drugs 9 14401468 [View Article] [PubMed] .
    [Google Scholar]
  12. Mansson M., Nielsen A., Kjærulff L., Gotfredsen C. H., Wietz M., Ingmer H., Gram L., Larsen T. O. ( 2011b;). Inhibition of virulence gene expression in Staphylococcus aureus by novel depsipeptides from a marine photobacterium . Mar Drugs 9 25372552 [View Article] [PubMed] .
    [Google Scholar]
  13. Meier-Kolthoff J. P., Auch A. F., Klenk H.-P., Göker M. ( 2013;). Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 14 60 [View Article] [PubMed] .
    [Google Scholar]
  14. Sambrook J., Russel D. W. ( 2001). Molecular Cloning: A Laboratory Manual Cold Spring Harbour, New York: Cold Spring Harbour Laboratory Press;.
    [Google Scholar]
  15. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: Molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed] .
    [Google Scholar]
  16. Wietz M., Mansson M., Gotfredsen C. H., Larsen T. O., Gram L. ( 2010;). Antibacterial compounds from marine Vibrionaceae isolated on a global expedition. Mar Drugs 8 29462960 [View Article] [PubMed] .
    [Google Scholar]
  17. ZoBell C. E. ( 1941;). Studies on Marine Bacteria. I. The cultural requirements of heterotrophic aerobes. J Mar Res 4 4275.
    [Google Scholar]
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