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Volume 65, Issue Pt_12

sp. nov., a moderately halophilic bacterium isolated from salterns Free

Abstract

Four bacterial strains, SN-14, SN-4, M6-46 and M6-58B, were isolated from water of ponds of two salterns located in Huelva (Spain). They were Gram-stain-negative, aerobic and slightly curved rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the four strains belong to the genus , being related most closely to F23 (98.4–98.0% sequence similarity), CL-SP19 (98.3–98.0%), TPS4-2 (97.9–97.4%), OS145 (97.5–97.4%) and KMM 231 (97.6–97.0%). The level of similarity with the type species of the genus, KMM 227, was 97.2–96.7%. The novel strains exhibited optimal growth at 5–10% (w/v) total salts, pH 7 and 37 °C. The major fatty acids of strain SN-14 were iso-C, iso-C, Cω7/Cω6, C and iso-Cω9/C 10-methyl. The DNA G+C content range was 47.6–50.8 mol%. The level of DNA–DNA relatedness between strain SN-14 and F23 was 13%, while those between strain SN-14 and the other three new isolates were between 77 and 99%. These data demonstrated that the four isolates constitute a novel species of the genus . Based on the phylogenetic, genotypic, phenotypic and chemotaxonomic data, the four strains represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SN-14 ( = CCM 8471 = CECT 8360 = LMG 27613).

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Funding
This study was supported by the:
  • Spanish Ministry of Science and Innovation (Award CGL2013-46941-P)
  • Junta de Andalucía (Award P10-CVI-6226)
© 2015 IUMS
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2015-12-01
2024-04-23
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Idiomarina aquatica sp. nov., a moderately halophilic bacterium isolated from salterns
Int J Syst Evol Microbiol 65, 4595 (2015); https://doi.org/10.1099/ijsem.0.000619
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