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Abstract

A polyphasic taxonomic study was undertaken to establish the status of a novel bacterium, designated strain WHSC-8, which was isolated from soil of Hengshui Lake Wetland Reserve in Hebei province, northern China. Colonies of this strain were yellow and cells were rod-shaped, polar-flagellated and obligately aerobic, exhibiting negative Gram reaction. The strain was able to grow at 0–1 % (w/v) NaCl, pH 5–10 and 20–35 °C, with optimal growth occurring at pH 7.0 and 28 °C without NaCl. Chemotaxonomic data revealed that strain WHSC-8 possesses ubiquinone Q-10 as the predominant respiratory quinone, Cω7, C and summed feature 3 (Cω7 and/or iso-C 2-OH) as the major fatty acids, and -homospermidine as the major polyamine. -specific sphingoglycolipid was detected in the polar lipid patterns. The G+C content of the genomic DNA was 68.7 mol%. All of the above characters corroborated the assignment of the novel strain to the genus . Strain WHSC-8 shared less than 97.0 % 16S rRNA gene sequence similarity with the type strains of other species of the genus , except for DSM 10564 (97.5 %). The low DNA–DNA relatedness value and distinct phenotypic and chemotaxonomic characteristics distinguished strain WHSC-8 from closely related species of the genus . Therefore, strain WHSC-8 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WHSC-8 ( = KCTC 42455 = CCTCC AB 2015265).

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2015-12-01
2024-03-28
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