RT Journal Article SR Electronic(1) A1 Wei, Shuzhen A1 Wang, Tingting A1 Liu, Hongliang A1 Zhang, Caifeng A1 Guo, Jiping A1 Wang, Qian A1 Liang, Kuijing A1 Zhang, ZhiqiangYR 2015 T1 Sphingomonas hengshuiensis sp. nov., isolated from lake wetland JF International Journal of Systematic and Evolutionary Microbiology, VO 65 IS Pt_12 SP 4644 OP 4649 DO https://doi.org/10.1099/ijsem.0.000626 PB Microbiology Society, SN 1466-5034, AB A polyphasic taxonomic study was undertaken to establish the status of a novel bacterium, designated strain WHSC-8T, which was isolated from soil of Hengshui Lake Wetland Reserve in Hebei province, northern China. Colonies of this strain were yellow and cells were rod-shaped, polar-flagellated and obligately aerobic, exhibiting negative Gram reaction. The strain was able to grow at 0–1 % (w/v) NaCl, pH 5–10 and 20–35 °C, with optimal growth occurring at pH 7.0 and 28 °C without NaCl. Chemotaxonomic data revealed that strain WHSC-8T possesses ubiquinone Q-10 as the predominant respiratory quinone, C18 : 1ω7c, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) as the major fatty acids, and sym-homospermidine as the major polyamine. Sphingomonadaceae-specific sphingoglycolipid was detected in the polar lipid patterns. The G+C content of the genomic DNA was 68.7 mol%. All of the above characters corroborated the assignment of the novel strain to the genus Sphingomonas. Strain WHSC-8T shared less than 97.0 % 16S rRNA gene sequence similarity with the type strains of other species of the genus Sphingomonas, except for Sphingomonas asaccharolytica DSM 10564T (97.5 %). The low DNA–DNA relatedness value and distinct phenotypic and chemotaxonomic characteristics distinguished strain WHSC-8T from closely related species of the genus Sphingomonas. Therefore, strain WHSC-8T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas hengshuiensis sp. nov. is proposed. The type strain is WHSC-8T ( = KCTC 42455T = CCTCC AB 2015265T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000626