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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 65, Issue Pt_12

sp. nov., isolated from soil Free

Abstract

A novel actinomycete, designated strain GK-6, was isolated from a soil sample from Nanning, Guangxi province, PR China. The strain grew at 20–40 °C, pH 6.0–11.0 and with 0–7.0 % NaCl. It formed well-developed aerial and vegetative mycelia. The aerial mycelium was white and the vegetative mycelium was yellow. The long branching aerial mycelia yielded rod-shaped arthrospores, the spores had smooth surfaces and were non-motile. Strain Gk-6 contained meso-diaminopimelic acid as the diagnostic diamino acid, the whole-cell sugars were galactose, glucose and arabinose. Major fatty acids were iso-C, iso-C and C. MK-9(H) was the predominant menaquinone. The polar phospholipids were phosphatidylethanolamine, phosphatidylethanolamine-containing hydroxylated fatty acids, diphosphatidylglycerol, ninhydrin-positive glycophospholipid and an unknown phospholipid. The G+C content of the genomic DNA was 73.4 mol%. The 16S rRNA gene sequence analysis indicated that the organism was a member of the genus and its closest relative among recognized species was JCM 17695 (97.7 % sequence similarity). But the phenotypic characteristics of strain Gk-6 were significantly different from those of JCM 17695, and DNA–DNA hybridization showed low relatedness (22.6–28.3 %) between strain Gk-6 and JCM 17695. On the basis of the phenotypic and phylogenetic data, strain Gk-6 represents a novel species of the genus , and the name sp. nov. is proposed. The type strain is Gk-6 ( = DSM 46779  = CGMCC 4.7154).

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Funding
This study was supported by the:
  • Guangxi Natural Science Foundation Key Programs (Award 2010GXNSFD013027)
  • Project of Guangxi Innovation Team of China Agriculture Research System (Award CARS-GXIT-08)
© 2015 IUMS
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2015-12-01
2024-04-18
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Actinokineospora guangxiensis sp. nov., isolated from soil
Int J Syst Evol Microbiol 65, 4650 (2015); https://doi.org/10.1099/ijsem.0.000627
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