1887

Abstract

A Gram-reaction-negative, aerobic, non-motile and rod-shaped bacterial strain designated Gsoil 636 was isolated from soil of a ginseng cultivation field in Pocheon Province, South Korea and its taxonomic position was investigated using a polyphasic approach. Gsoil 636 grew at 18–30 °C and at pH 6.0–8.0 on R2A medium. Gsoil 636 possessed β-glucosidase activity, which was responsible for its ability to transform ginsenoside Rb (ones of the dominant active components of ginseng) to F. On the basis of 16S rRNA gene sequence similarity, Gsoil 636 was shown to belong to the family and to be related to Gsoil 492 (96.7 % sequence similarity), Gsoil 643 (96.6 %) and 02SUJ3 (96.6 %). The G+C content of the genomic DNA was 48.9 %. The predominant respiratory quinone was MK-7 and the major fatty acids were iso-C, summed feature 3 (comprising Cω6 and/or Cω7) and iso-C 3-OH. DNA and chemotaxonomic data supported the affiliation of Gsoil 636 to the genus . Gsoil 636 could be differentiated genotypically and phenotypically from the species of the genus with validly published names. The isolate therefore represents a novel species, for which the name sp. nov. is proposed, with the type strain Gsoil 636 (KCTC 22818 = JCM 18197 = KACC 14277).

Funding
This study was supported by the:
  • National Institute of Biological Resources (NIBR)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000660
2015-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4868.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000660&mimeType=html&fmt=ahah

References

  1. Atlas R. M. ( 1993). Handbook of Microbiological Media Boca Raton: CRC Press;.
    [Google Scholar]
  2. Baik K. S., Kim M. S., Lee J. H., Lee S. S., Im W. T., Seong C. N. ( 2014;). Flavisolibacter rigui sp. nov., isolated from freshwater of an artificial reservoir and emended description of the genus Flavisolibacter . Int J Syst Evol Microbiol 64 40384042 [View Article] [PubMed] .
    [Google Scholar]
  3. Buck J. D. ( 1982;). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. Appl Environ Microbiol 44 992993 [PubMed].
    [Google Scholar]
  4. Cappuccino J. G., Sherman N. ( 2002). Microbiology: a Laboratory Manual , 6th edn.., California: Pearson Education, Inc;.
    [Google Scholar]
  5. Chang K. H., Jo M. N., Kim K. T., Paik H. D. ( 2014;). Evaluation of glucosidases of Aspergillus niger strain comparing with other glucosidases in transformation of ginsenoside Rb1 to ginsenosides Rg3 . J Ginseng Res 38 4751 [View Article] [PubMed] .
    [Google Scholar]
  6. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  7. Fitch W. M. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20 406416 [View Article].
    [Google Scholar]
  8. Hall T. A. ( 1999;). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41 9598.
    [Google Scholar]
  9. Hiraishi A., Ueda Y., Ishihara J., Mori T. ( 1996;). Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 42 457469 [View Article].
    [Google Scholar]
  10. Im W.-T., Kim S.-Y., Liu Q.-M., Yang J.-E., Lee S.-T., Yi T.-H. ( 2010;). Nocardioides ginsengisegetis sp. nov., isolated from soil of a ginseng field. J Microbiol 48 623628 [View Article] [PubMed] .
    [Google Scholar]
  11. Kämpfer P., Lodders N., Falsen E. ( 2011;). Hydrotalea flava gen. nov., sp. nov., a new member of the phylum Bacteroidetes and allocation of the genera Chitinophaga, Sediminibacterium, Lacibacter, Flavihumibacter, Flavisolibacter, Niabella, Niastella, Segetibacter, Parasegetibacter, Terrimonas, Ferruginibacter, Filimonas, Hydrotalea to the family Chitinophagaceae fam. nov. Int J Syst Evol Microbiol 61 518523 [View Article] [PubMed] .
    [Google Scholar]
  12. Kim O. S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H., other authors. ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62 716721 [View Article] [PubMed] .
    [Google Scholar]
  13. Kim M. J., Yun H., Kim D. H., Kang I., Choe W., Kim S. S., Ha J. ( 2014;). AMP-activated protein kinase determines apoptotic sensitivity of cancer cells to ginsenoside-Rh2 . J Ginseng Res 38 1621 [View Article] [PubMed] .
    [Google Scholar]
  14. Kimura M. ( 1983). The Neutral Theory of Molecular Evolution [View Article] Cambridge University Press;.
    [Google Scholar]
  15. Mesbah M., Premachandran U., Whitman W. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Evol Microbiol 39 159167.
    [Google Scholar]
  16. Moore D. D., Dowhan D. ( 1995;). Preparation and Analysis of DNA. . In Current Protocols in Molecular Biology, pp. 211. Edited by Ausubel F. W., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley;.
    [Google Scholar]
  17. Park C. S., Han K., Ahn T. Y. ( 2014;). Mucilaginibacter koreensis sp. nov., isolated from leaf mould. Int J Syst Evol Microbiol 64 22742279 [View Article] [PubMed] .
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  19. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  20. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. ( 2013;). mega6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol 30 27252729 [View Article] [PubMed] .
    [Google Scholar]
  21. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25 48764882 [View Article] [PubMed] .
    [Google Scholar]
  22. Weisburg W. G., Barns S. M., Pelletier D. A., Lane D. J. ( 1991;). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173 697703 [PubMed].
    [Google Scholar]
  23. Yoon M. H., Im W. T. ( 2007;). Flavisolibacter ginsengiterrae gen. nov., sp. nov. and Flavisolibacter ginsengisoli sp. nov., isolated from ginseng cultivating soil. Int J Syst Evol Microbiol 57 18341839 [View Article] [PubMed] .
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000660
Loading
/content/journal/ijsem/10.1099/ijsem.0.000660
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error