1887

Abstract

A novel actinomycete, designated strain 2902at01 was isolated from soil collected at a mangrove forest in Zhanjiang, Guangdong province, China. The strain was identified using a polyphasic classification method. The 16S rRNA gene sequence of strain 2902at01 showed the highest similarity to Y22 (98.3 %) and TJ2-2 (98.1 %). Phylogenetic analysis based on the gene sequence also clearly showed that the strain was different from any previously discovered species of the genus . The characteristic whole-cell sugars were ribose and xylose. The cell-wall hydrolysates contained alanine, asparagine, glycine and -diaminopimelic acid. MK-10(H) and MK-10(H) were the major menaquinones of the novel strain. The predominant fatty acids were iso-C, anteiso-C and iso-C The characteristic polar lipids of strain 2902at01 were phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside and diphosphatidylglycerol. The DNA G+C content was 70.2 mol%. DNA–DNA hybridization data combined with other physiological and biochemical features could distinguish strain 2902at01 from the reference strains Y22 and TJ2-2 . On the basis of these phenotypic and genotypic data, strain 2902at01 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 2902at01 ( = CCTCC AA2014018 = DSM 45902).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000667
2015-12-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/65/12/4880.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000667&mimeType=html&fmt=ahah

References

  1. Arai T. ( 1975). Culture Media for Actinomycetes., Tokyo: The Society for Actinomycetes Japan;.
    [Google Scholar]
  2. Carro L., Pukall R., Spröer C., Kroppenstedt R. M., Trujillo M. E. ( 2012;). Micromonospora cremea sp. nov. and Micromonospora zamorensis sp. nov., isolated from the rhizosphere of Pisum sativum. Int J Syst Evol Microbiol 62 29712977 [View Article] [PubMed] .
    [Google Scholar]
  3. Castiglione F., Lazzarini A., Carrano L., Corti E., Ciciliato I., Gastaldo L., Candiani P., Losi D., Marinelli F., other authors. ( 2008;). Determining the structure and mode of action of microbisporicin, a potent lantibiotic active against multiresistant pathogens. Chem Biol 15 2231 [View Article] [PubMed] .
    [Google Scholar]
  4. Costanza R., d'Arge R., de Groot R., Farber S., Grasso M., Hannon B., Limburg K., Naeem S., O'Neill R. V., other authors. ( 1997;). The value of the world's ecosystem services and natural capital. Nature 387 253260 [View Article].
    [Google Scholar]
  5. Everest G. J., Meyers P. R. ( 2013;). Micromonospora equina sp. nov., isolated from soil from a racecourse. Int J Syst Evol Microbiol 63 879885 [View Article] [PubMed] .
    [Google Scholar]
  6. Felsenstein J. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17 368376 [View Article] [PubMed] .
    [Google Scholar]
  7. Felsenstein J. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 783791 [View Article].
    [Google Scholar]
  8. Garcia L. C., Martínez-Molina E., Trujillo M. E. ( 2010;). Micromonospora pisi sp. nov., isolated from root nodules of Pisum sativum. Int J Syst Evol Microbiol 60 331337 [View Article] [PubMed] .
    [Google Scholar]
  9. Hong K. ( 2013;). [Actinomycetes from mangrove and their secondary metabolites]. Wei Sheng Wu Xue Bao 53 11311141 . (In Chinese) .
    [Google Scholar]
  10. Hong K., Gao A. H., Xie Q. Y., Gao H., Zhuang L., Lin H. P., Yu H. P., Li J., Yao X. S., other authors. ( 2009;). Actinomycetes for marine drug discovery isolated from mangrove soils and plants in China. Mar Drugs 7 2444 [View Article] [PubMed] .
    [Google Scholar]
  11. Huang H., Lv J., Hu Y., Fang Z., Zhang K., Bao S. ( 2008;). Micromonospora rifamycinica sp. nov., a novel actinomycete from mangrove sediment. Int J Syst Evol Microbiol 58 1720 [View Article] [PubMed] .
    [Google Scholar]
  12. Kämpfer P., Steiof M., Dott W. ( 1991;). Microbiological characterization of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 21 227251 [View Article] [PubMed] .
    [Google Scholar]
  13. Kawamoto I. ( 1989;). Genus Micromonospora Ørskov 1923 147AL . . In Bergey's Manual of Systematic Bacteriology, pp. 24422450. Edited by Williams S. T., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;.
    [Google Scholar]
  14. Kelly K. L. ( 1964). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors., Washington, DC: US Government Printing Office;.
    [Google Scholar]
  15. Kluge A. G., Farris J. S. ( 1969;). Quantitative phyletics and the evolution of anurans. Syst Zool 18 132 [View Article].
    [Google Scholar]
  16. Kroppenstedt R. M. ( 1985;). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173199. Edited by Goodfellow M., Minnikin D. E. New York: Academic Press;.
    [Google Scholar]
  17. Lechevalier M. P., Lechevalier H. A. ( 1980;). The chemotaxonomy of actinomycetes. . In Actinomycete Taxonomy (Special Publication no. 6), pp. 227291. Edited by Dietz A., Thayer D. W. Arlington, VA: Society of Industrial Microbiology;.
    [Google Scholar]
  18. Lechevalier M. P., De Bièvre C., Lechevalier H. A. ( 1977;). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5 249260 [View Article].
    [Google Scholar]
  19. Li L., Mao Y. J., Xie Q. Y., Deng Z., Hong K. ( 2013a;). Micromonospora avicenniae sp. nov., isolated from a root of Avicennia marina . Antonie van Leeuwenhoek 103 10891096 [View Article] [PubMed] .
    [Google Scholar]
  20. Li L., Tang Y. L., Wei B., Xie Q. Y., Deng Z., Hong K. ( 2013b;). Micromonospora sonneratiae sp. nov., isolated from a root of Sonneratia apetala. Int J Syst Evol Microbiol 63 23832388 [View Article] [PubMed] .
    [Google Scholar]
  21. Mesbah M., Premachandran U., Whitman W. B. ( 1989;). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39 159167 [View Article].
    [Google Scholar]
  22. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984;). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2 233241 [View Article].
    [Google Scholar]
  23. Nakajima Y., Kitpreechavanich V., Suzuki K., Kudo T. ( 1999;). Microbispora corallina sp. nov., a new species of the genus Microbispora isolated from Thai soil. Int J Syst Bacteriol 49 17611767 [View Article] [PubMed] .
    [Google Scholar]
  24. Ørskov J. ( 1923). Investigations into the Morphology of the Ray Fungi Copenhagen: Levin and Munksgaard;.
    [Google Scholar]
  25. Pospiech A., Neumann B. ( 1995;). A versatile quick-prep of genomic DNA from Gram-positive bacteria. Trends Genet 11 217218 [View Article] [PubMed] .
    [Google Scholar]
  26. Saitou N., Nei M. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4 406425 [PubMed].
    [Google Scholar]
  27. Sasser M. ( 1990). Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;.
    [Google Scholar]
  28. Shirling E. B., Gottlieb D. ( 1966;). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16 313340 [View Article].
    [Google Scholar]
  29. Songsumanus A., Tanasupawat S., Igarashi Y., Kudo T. ( 2013;). Micromonospora maritima sp. nov., isolated from mangrove soil. Int J Syst Evol Microbiol 63 554559 [View Article] [PubMed] .
    [Google Scholar]
  30. Stackebrandt E., Rainey F. A., Ward-Rainey N. L. ( 1997;). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47 479491 [View Article].
    [Google Scholar]
  31. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28 27312739 [View Article] [PubMed] .
    [Google Scholar]
  32. Teather R. M., Wood P. J. ( 1982;). Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl Environ Microbiol 43 777780 [PubMed].
    [Google Scholar]
  33. Thawai C., Tanasupawat S., Kudo T. ( 2008;). Micromonospora pattaloongensis sp. nov., isolated from a Thai mangrove forest. Int J Syst Evol Microbiol 58 15161521 [View Article] [PubMed] .
    [Google Scholar]
  34. Trujillo M. E., Fernández-Molinero C., Velázquez E., Kroppenstedt R. M., Schumann P., Mateos P. F., Martínez-Molina E. ( 2005;). Micromonospora mirobrigensis sp. nov. Int J Syst Evol Microbiol 55 877880 [View Article] [PubMed] .
    [Google Scholar]
  35. Waksman S. A. ( 1967). The Actinomycetes. A Summary of Current Knowledge., New York: Ronald Press;.
    [Google Scholar]
  36. Wang C., Xu X. X., Qu Z., Wang H. L., Lin H. P., Xie Q. Y., Ruan J. S., Hong K. ( 2011;). Micromonospora rhizosphaerae sp. nov., isolated from mangrove rhizosphere soil. Int J Syst Evol Microbiol 61 320324 [View Article] [PubMed] .
    [Google Scholar]
  37. Williams S. T., Cross T. ( 1971;). Actinomycetes. Methods Microbiol 4 295334 [View Article].
    [Google Scholar]
  38. Williams S. T., Goodfellow M., Alderson G., Wellington E.M.H., Sneath P.H.A., Sackin M. J. ( 1983;). Numerical classification of Streptomyces and related genera. J Gen Microbiol 129 17431813 [PubMed].
    [Google Scholar]
  39. Xie Q. Y., Wang C., Wang R., Qu Z., Lin H. P., Goodfellow M., Hong K. ( 2011;). Jishengella endophytica gen. nov., sp. nov., a new member of the family Micromonosporaceae. Int J Syst Evol Microbiol 61 11531159 [View Article] [PubMed] .
    [Google Scholar]
  40. Xie Q. Y., Qu Z., Lin H. P., Li L., Hong K. ( 2012;). Micromonospora haikouensis sp. nov., isolated from mangrove soil. Antonie van Leeuwenhoek 101 649655 [View Article] [PubMed] .
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000667
Loading
/content/journal/ijsem/10.1099/ijsem.0.000667
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error