1887

Abstract

Biochemical and molecular genetic studies were performed on three novel Gram-stain-negative, catalase- and oxidase-positive, bacilli-shaped organisms isolated from the tonsils of two pigs and one wild boar. The micro-organism was identified as a species of the genus based on its cellular morphological and biochemical tests. The closest phylogenetic relative of the novel bacilli was HM-7 (98.2 % 16S rRNA gene sequence similarity to the type strain). and sequence analysis showed interspecies divergence from the closest 16S rRNA gene phylogenetic relative, of 87.0.% and 69 %, respectively. The polyamine pattern contains predominantly putrescine and 2-hydroxyputrescine. The major quinone is ubiquinone Q-8 and in the polar lipid profile, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminolipid and an unidentified lipid are predominant. The novel bacterial isolate can be distinguished from by several biochemical characteristics, such as the production of -pyrrolydonil arylamidase but not gamma-glutamyl-transferase, and the utilization of different carbon sources. Based on both phenotypic and phylogenetic findings, the novel bacterium is classified as representing a novel species of the genus for which the name sp. nov. is proposed. The type strain is 3340-03 ( = CECT 8400 = CCUG 64465).

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2015-12-01
2024-03-28
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