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Volume 66, Issue 1

sp. nov., isolated from a sea squirt () Free

Abstract

A Gram-stain-negative, non-spore-forming, non-flagellated and coccoid, ovoid or rod-shaped bacterial strain, RA1-3, was isolated from a sea squirt () collected from the South Sea, South Korea, and subjected to a taxonomic study using a polyphasic approach. Strain RA1-3 grew optimally at 25 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. Neighbour-joining, maximum-likelihood and maximum-parsimony phylogenetic trees based on 16S rRNA gene sequences revealed that strain RA1-3 clustered with the type strains of three species of the genus , showing 97.54–98.41 % 16S rRNA gene sequence similarity. Sequence similarities to other recognized species were less than 96.97 %. Strain RA1-3 contained Q-10 as the predominant ubiquinone and Cω7 as the major fatty acid. The major polar lipids of strain RA1-3 were phosphatidylcholine, phosphatidylglycerol, one unidentified aminolipid and one unidentified lipid. The DNA G+C content of strain RA1-3 was 56 mol% and DNA–DNA relatedness values with the type strains of , and were 13–24 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain RA1-3 is separated from other recognized species of the genus . On the basis of the data presented, strain RA1-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RA1-3 ( = KCTC 42605 = CECT 8868).

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2016-01-01
2024-04-23
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References

  1. Barrow G. I., Feltham R.K.A.(editors). 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn.. Cambridge: Cambridge University Press; [View Article]
     [Google Scholar]
  2. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . In The Prokaryotes pp 1302–1331Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
     [Google Scholar]
  3. Billerbeck S., Orchard J., Tindall B. J., Giebel H.-A., Brinkhoff T., Simon M. 2015; Description of Octadecabacter temperatus sp. nov., isolated from the southern North Sea, emended descriptions of the genus Octadecabacter and its species and reclassification of Octadecabacter jejudonensis Park and Yoon 2014 as Pseudooctadecabacter jejudonensis gen. nov., comb. nov. Int J Syst Evol Microbiol 65:1967–1974 [View Article][PubMed]
     [Google Scholar]
  4. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
     [Google Scholar]
  5. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49:25–68 [View Article][PubMed]
     [Google Scholar]
  6. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. In Modern Microbial Methods. Chemical Methods in Prokaryotic Systematics pp 121–161Edited by Goodfellow M., O'Donnell A. G. Chichester: John Wiley & Sons;
     [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
     [Google Scholar]
  8. Gosink J. J., Herwig R. P., Staley J. T. 1997; Octadecabacter arcticus gen. nov., sp. nov., and O. antarcticus, sp. nov., nonpigmented, psychrophilic gas vacuolate bacteria from polar sea ice and water. Syst Appl Microbiol 20:356–365 [View Article]
     [Google Scholar]
  9. Gosink J. J., Herwig R. P., Staley J. T. 1998; Octadecabacter gen. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, Validation List 64. Int J Syst Bacteriol 48:327–328 [View Article]
     [Google Scholar]
  10. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
     [Google Scholar]
  11. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
     [Google Scholar]
  12. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
     [Google Scholar]
  13. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
     [Google Scholar]
  14. Park S., Yoon J.-H. 2014; Octadecabacter jejudonensis sp. nov., isolated from the junction between the ocean and a freshwater spring and emended description of the genus Octadecabacter . Int J Syst Evol Microbiol 64:719–724 [View Article][PubMed]
     [Google Scholar]
  15. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
     [Google Scholar]
  16. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
     [Google Scholar]
  17. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942[PubMed]
     [Google Scholar]
  18. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
     [Google Scholar]
  19. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
     [Google Scholar]
  20. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
     [Google Scholar]
  21. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
     [Google Scholar]
  22. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
     [Google Scholar]
  23. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [View Article][PubMed]
     [Google Scholar]
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Octadecabacter ascidiaceicola sp. nov., isolated from a sea squirt (Halocynthia roretzi)
Int J Syst Evol Microbiol 66, 296 (2016); https://doi.org/10.1099/ijsem.0.000715
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