%0 Journal Article %A Zhang, Lei %A Gao, Ju-Sheng %A Kim, Song-Gun %A Zhang, Cai-Wen %A Jiang, Ju-Quan %A Ma, Xiao-Tong %A Zhang, Jun %A Zhang, Xiao-Xia %T Novosphingobium oryzae sp. nov., a potential plant-promoting endophytic bacterium isolated from rice roots %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 1 %P 302-307 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.000718 %I Microbiology Society, %X A novel endophytic bacterium, strain ZYY112T, isolated from rice roots, was characterized by a polyphasic approach. In phylogenetic analyses based on 16S rRNA gene sequences, ZYY112T showed highest sequence similarity to Novosphingobium sediminicola HU1-AH51T (97.2 %) and less than 97 % similarity with respect to other Novosphingobium species with validly published names. The DNA G+C content of strain ZYY112T was 60.8 mol%. The level of DNA–DNA relatedness between strain ZYY112T and N. sediminicola DSM 27057T was 33.7 % (reciprocal 5.2 %), which supported the suggestion that ZYY112T represented a novel species of the genus Novosphingobium. Ubiquinone Q-10 was the unique respiratory quinone (100 %). The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, an unknown aminolipid and an unknown phospholipid. The major fatty acids of strain ZYY112T were summed feature 8 (consisting of C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (consisting of C16 : 1ω7c and/or C16 : 1ω6c), C14 : 0 2-OH and C16 : 0. The major polyamine of ZYY112T was spermidine, which is a characteristic trait of the genus Novosphingobium. Characterization by genotypic, chemotaxonomic and phenotypic analysis indicated that strain ZYY112T represents a novel species of the genus Novosphingobium, for which the name Novosphingobium oryzae sp. nov. is proposed. The type strain is ZYY112T ( = ACCC 06131T = JCM 30537T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000718