RT Journal Article SR Electronic(1) A1 Zhang, Lei A1 Gao, Ju-Sheng A1 Kim, Song-Gun A1 Zhang, Cai-Wen A1 Jiang, Ju-Quan A1 Ma, Xiao-Tong A1 Zhang, Jun A1 Zhang, Xiao-XiaYR 2016 T1 Novosphingobium oryzae sp. nov., a potential plant-promoting endophytic bacterium isolated from rice roots JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 1 SP 302 OP 307 DO https://doi.org/10.1099/ijsem.0.000718 PB Microbiology Society, SN 1466-5034, AB A novel endophytic bacterium, strain ZYY112T, isolated from rice roots, was characterized by a polyphasic approach. In phylogenetic analyses based on 16S rRNA gene sequences, ZYY112T showed highest sequence similarity to Novosphingobium sediminicola HU1-AH51T (97.2 %) and less than 97 % similarity with respect to other Novosphingobium species with validly published names. The DNA G+C content of strain ZYY112T was 60.8 mol%. The level of DNA–DNA relatedness between strain ZYY112T and N. sediminicola DSM 27057T was 33.7 % (reciprocal 5.2 %), which supported the suggestion that ZYY112T represented a novel species of the genus Novosphingobium. Ubiquinone Q-10 was the unique respiratory quinone (100 %). The polar lipid profile contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, an unknown aminolipid and an unknown phospholipid. The major fatty acids of strain ZYY112T were summed feature 8 (consisting of C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (consisting of C16 : 1ω7c and/or C16 : 1ω6c), C14 : 0 2-OH and C16 : 0. The major polyamine of ZYY112T was spermidine, which is a characteristic trait of the genus Novosphingobium. Characterization by genotypic, chemotaxonomic and phenotypic analysis indicated that strain ZYY112T represents a novel species of the genus Novosphingobium, for which the name Novosphingobium oryzae sp. nov. is proposed. The type strain is ZYY112T ( = ACCC 06131T = JCM 30537T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000718