1887

Abstract

Two Gram-negative, aerobic, non-motile, rod-shaped bacterial strains, FH13 and FH23, representing a novel group of isolated from root nodules of in Mexico, were studied by a polyphasic analysis. Phylogeny of 16S rRNA gene sequences revealed them to be members of the genus related most closely to ‘’ CCBAU 23252 (99.7 % similarity), USDA 2370 (98.6 %), and CCBAU 03386 and others ( ≤ 98.3 %). In sequence analyses of the housekeeping genes and both strains formed a subclade distinct from all defined species of the genus at sequence similarities of 82.3–94.0 %, demonstrating that they represented a novel genomic species in the genus . Mean levels of DNA–DNA relatedness between the reference strain FH13 and the type strains of related species varied between 13.0 ± 2.0 and 52.1 ± 1.2 %. The DNA G+C content of strain FH13 was 63.5 mol% ( ). The major cellular fatty acids were 16 : 0, 17 : 0 anteiso, 18 : 0, summed feature 2 (12 : 0 aldehyde/unknown 10.928) and summed feature 8 (18 : 1ω7). The fatty acid 17 : 1ω5 was unique for this strain. Some phenotypic features, such as failure to utilize adonitol, -arabinose, -fructose and -fucose, and ability to utilize -galacturonic acid and itaconic acid as carbon source, could also be used to distinguish strain FH13 from the type strains of related species. Based upon these results, a novel species, sp. nov., is proposed, with FH13 ( = CCBAU 101094 = HAMBI 3626 = LMG 28672) as the type strain.

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2016-01-01
2024-03-28
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