%0 Journal Article %A Román-Ponce, Brenda %A Jing Zhang, Yu %A Soledad Vásquez-Murrieta, María %A Hua Sui, Xin %A Feng Chen, Wen %A Carlos Alberto Padilla, Juan %A Wu Guo, Xian %A Lian Gao, Jun %A Yan, Jun %A Hong Wei, Ge %A Tao Wang, En %T Rhizobium acidisoli sp. nov., isolated from root nodules of Phaseolus vulgaris in acid soils %D 2016 %J International Journal of Systematic and Evolutionary Microbiology, %V 66 %N 1 %P 398-406 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.000732 %I Microbiology Society, %X Two Gram-negative, aerobic, non-motile, rod-shaped bacterial strains, FH13T and FH23, representing a novel group of Rhizobium isolated from root nodules of Phaseolus vulgaris in Mexico, were studied by a polyphasic analysis. Phylogeny of 16S rRNA gene sequences revealed them to be members of the genus Rhizobium related most closely to ‘Rhizobium anhuiense’ CCBAU 23252 (99.7 % similarity), Rhizobium leguminosarum USDA 2370T (98.6 %), and Rhizobium sophorae CCBAU 03386T and others ( ≤ 98.3 %). In sequence analyses of the housekeeping genes recA, glnII and atpD, both strains formed a subclade distinct from all defined species of the genus Rhizobium at sequence similarities of 82.3–94.0 %, demonstrating that they represented a novel genomic species in the genus Rhizobium. Mean levels of DNA–DNA relatedness between the reference strain FH13T and the type strains of related species varied between 13.0 ± 2.0 and 52.1 ± 1.2 %. The DNA G+C content of strain FH13T was 63.5 mol% (T m). The major cellular fatty acids were 16 : 0, 17 : 0 anteiso, 18 : 0, summed feature 2 (12 : 0 aldehyde/unknown 10.928) and summed feature 8 (18 : 1ω7c). The fatty acid 17 : 1ω5c was unique for this strain. Some phenotypic features, such as failure to utilize adonitol, l-arabinose, d-fructose and d-fucose, and ability to utilize d-galacturonic acid and itaconic acid as carbon source, could also be used to distinguish strain FH13T from the type strains of related species. Based upon these results, a novel species, Rhizobium acidisoli sp. nov., is proposed, with FH13T ( = CCBAU 101094T = HAMBI 3626T = LMG 28672T) as the type strain. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000732