1887

Abstract

A Gram-stain-positive, facultatively anaerobic bacterial strain, designated WLJ055, with polar and subpolar flagella was isolated from the top layer of desert soil from Erdos, Inner Mongolia, northern China. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain WLJ055 was a member of the genus , and shared 97.17–97.24 % 16S rRNA gene sequence similarities with GR21 and HR1. The major polar lipids of strain WLJ055 were diphosphatidylglycerol, phosphatidylglycerol, an unknown aminophospholipid, two unknown glycolipids and an unknown phosphoglycolipid. MK-7 was the predominant menaquinone, while anteiso-C, C, iso-C, and anteiso-C were the major cellular fatty acids. Its genomic DNA G+C content was 55.5 mol%. DNA–DNA hybridization revealed that strain WLJ055 showed 45 ± 5 % and 40 ± 5 % genomic DNA relatedness with its two closest relatives, GR21 and HR1, respectively. The results of physiological and biochemical tests allowed the discrimination of strain WLJ055 from its phylogenetic relatives. sp. nov. is therefore proposed to be a novel species of the genus , with strain WLJ055 ( = CGMCC 1.15276 = KCTC 33693) as the type strain.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000766
2016-02-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/2/623.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000766&mimeType=html&fmt=ahah

References

  1. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  2. Dong X. Z., Cai M. Y. 2001 Determinative Manual for Routine Bacteriology Beijing: Science Press; (English translation)
    [Google Scholar]
  3. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  4. Felsenstein J. 1985; Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791 [View Article]
    [Google Scholar]
  5. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  6. Fraser S. L., Jorgensen J. H. 1997; Reappraisal of the antimicrobial susceptibilities of Chryseobacterium and Flavobacterium species and methods for reliable susceptibility testing. Antimicrob Agents Chemother 41:2738–2741[PubMed]
    [Google Scholar]
  7. Huss V. A., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
    [Google Scholar]
  8. Kates M. 1986 Techniques of Lipidology: Isolation, Analysis and Identification of Lipids, 2nd edn. Amsterdam: Elsevier;
    [Google Scholar]
  9. Kim B.-C., Jeong W.-J., Oh H.-W., Kim H., Park D.-S., Park H.-M., Bae K. S. 2009; Paenibacillus pueri sp. nov., isolated from Pu'er tea. Int J Syst Evol Microbiol 59:1002–1006 [View Article][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  11. Komagata K., Suzuki K. 1987; Lipid and cell wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  12. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B195–206 [View Article]
    [Google Scholar]
  13. Rivas R., García-Fraile P., Zurdo-Piñeiro J. L., Mateos P. F., Martínez-Molina E., Bedmar E. J., Sánchez-Raya J., Velázquez E. 2008; Saccharibacillus sacchari gen. nov., sp. nov., isolated from sugar cane. Int J Syst Evol Microbiol 58:1850–1854 [View Article][PubMed]
    [Google Scholar]
  14. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  15. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101. Newark, DE: MIDI Inc;
    [Google Scholar]
  16. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  17. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reasscocitation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  18. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013; mega6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729 [View Article][PubMed]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  20. Wang Y.-N., Cai H., Yu S.-L., Wang Z.-Y., Liu J., Wu X.-L. 2007; Halomonas gudaonensis sp. nov., isolated from a saline soil contaminated by crude oil. Int J Syst Evol Microbiol 57:911–915 [View Article][PubMed]
    [Google Scholar]
  21. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D, Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C, Murray R. G. E, other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  22. Yang S.-Y., Liu H., Liu R., Zhang K.-Y., Lai R. 2009; Saccharibacillus kuerlensis sp. nov., isolated from a desert soil. Int J Syst Evol Microbiol 59:953–957 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000766
Loading
/content/journal/ijsem/10.1099/ijsem.0.000766
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error