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Abstract

A Gram-stain-negative, non-flagellated, non-spore-forming bacterial strain motile by gliding, designated RSS1-6, was isolated from a golden sea squirt and its taxonomic position was investigated by using a polyphasic approach. Strain RSS1-6 grew optimally at 30–37 °C and in the presence of 1.0–4.0 % (w/v) NaCl. Phylogenetic trees based on 16S rRNA gene sequences showed that strain RSS1-6 fell within the clade comprising species of the genus , clustering with the type strains of , and with which it exhibited 16S rRNA gene sequence similarity values of 98.5–99.5 %. Strain RSS1-6 contained MK-6 as the predominant menaquinone and iso-C, iso-C 3-OH and summed feature 3 (Cω7 and/or Cω6) as the major fatty acids. The major polar lipids of strain RSS1-6 were phosphatidylethanolamine, two unidentified lipids, one unidentified aminophospholipid and one unidentified glycolipid. The DNA G+C content was 32.5 mol% and the mean DNA–DNA relatedness values with the type strains of , and were 17.3–25.2 %. The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness, revealed that strain RSS1-6 is separated from other recognized species of the genus . On the basis of the data presented, strain RSS1-6 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RSS1-6 ( = KCTC 42702 = NBRC 111225).

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2016-03-01
2024-03-29
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References

  1. Barrow G. I., Feltham R. K. A. editors 1993 Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press; [View Article]
    [Google Scholar]
  2. Baumann P., Baumann L. 1981; The marine Gram-negative eubacteria: genera Photobacterium, Beneckea, Alteromonas, Pseudomonas, and Alcaligenes . In The Prokaryotes pp 1302–1331Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. Berlin: Springer;
    [Google Scholar]
  3. Bernardet J.-F. 2011; Family I. Flavobacteriaceae Reichenbach 1992. In Bergey's Manual of Systematic Bacteriology, 2nd edn. pp 106–111Edited by Krieg N. R., Staley J. T., Brown D. R., Hedlund B. P., Paster B. J., Ward N. L., Ludwig W., Whitman W. B. New York: Springer;
    [Google Scholar]
  4. Bernardet J.-F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes 2002; Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52:1049–1070[PubMed]
    [Google Scholar]
  5. Bowman J. P. 2000; Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868 [View Article][PubMed]
    [Google Scholar]
  6. Bruns A., Rohde M., Berthe-Corti L. 2001; Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51:1997–2006 [View Article][PubMed]
    [Google Scholar]
  7. Choi D. H., Kim Y.-G., Hwang C. Y., Yi H., Chun J., Cho B. C. 2006; Tenacibaculum litoreum sp. nov., isolated from tidal flat sediment. Int J Syst Evol Microbiol 56:635–640 [View Article][PubMed]
    [Google Scholar]
  8. Cohen-Bazire G., Sistrom W. R., Stanier R. Y. 1957; Kinetic studies of pigment synthesis by non-sulfur purple bacteria. J Cell Physiol 49:25–68 [View Article][PubMed]
    [Google Scholar]
  9. Embley T. M., Wait R. 1994; Structural lipids of eubacteria. In Chemical Methods in Prokaryotic Systematics pp 121–161Edited by Goodfellow M., O'Donnell A. G. Chichester: Wiley;
    [Google Scholar]
  10. Ezaki T., Hashimoto Y., Yabuuchi E. 1989; Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39:224–229 [View Article]
    [Google Scholar]
  11. Frette L., Jørgensen N. O. G., Irming H., Kroer N. 2004; Tenacibaculum skagerrakense sp. nov., a marine bacterium isolated from the pelagic zone in Skagerrak, Denmark. Int J Syst Evol Microbiol 54:519–524 [View Article][PubMed]
    [Google Scholar]
  12. Heindl H., Wiese J., Imhoff J. F. 2008; Tenacibaculum adriaticum sp. nov., from a bryozoan in the Adriatic Sea. Int J Syst Evol Microbiol 58:542–547 [View Article][PubMed]
    [Google Scholar]
  13. Jung S.-Y., Oh T.-K., Yoon J.-H. 2006; Tenacibaculum aestuarii sp. nov., isolated from a tidal flat sediment in Korea. Int J Syst Evol Microbiol 56:1577–1581 [View Article][PubMed]
    [Google Scholar]
  14. Kang S.-J., Lee S.-Y., Lee M.-H., Oh T.-K., Yoon J.-H. 2012; Tenacibaculum geojense sp. nov., isolated from seawater. Int J Syst Evol Microbiol 62:18–22 [View Article][PubMed]
    [Google Scholar]
  15. Kim M., Oh H.-S., Park S.-C., Chun J. 2014; Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 64:346–351 [View Article][PubMed]
    [Google Scholar]
  16. Komagata K., Suzuki K. 1987; Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19:161–207 [View Article]
    [Google Scholar]
  17. Lányí B. 1987; Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19:1–67 [View Article]
    [Google Scholar]
  18. Lee Y. S., Baik K. S., Park S. Y., Kim E. M., Lee D.-H., Kahng H.-Y., Jeon C. O., Jung J. S. 2009; Tenacibaculum crassostreae sp. nov., isolated from the Pacific oyster, Crassostrea gigas . Int J Syst Evol Microbiol 59:1609–1614 [View Article][PubMed]
    [Google Scholar]
  19. Leifson E. 1963; Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85:1183–1184[PubMed]
    [Google Scholar]
  20. Li Y., Wei J., Yang C., Lai Q., Chen Z., Li D., Zhang H., Tian Y., Zheng W., Zheng T. 2013; Tenacibaculum xiamenense sp. nov., an algicidal bacterium isolated from coastal seawater. Int J Syst Evol Microbiol 63:3481–3486 [View Article][PubMed]
    [Google Scholar]
  21. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H.-P. 2013; When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195:413–418 [View Article][PubMed]
    [Google Scholar]
  22. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. 1984; An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2:233–241 [View Article]
    [Google Scholar]
  23. Oh Y.-S., Kahng H.-Y., Lee D.-H., Lee S. B. 2012; Tenacibaculum jejuense sp. nov., isolated from coastal seawater. Int J Syst Evol Microbiol 62:414–419 [View Article][PubMed]
    [Google Scholar]
  24. Park S., Yoon J.-H. 2013; Tenacibaculum caenipelagi sp. nov., a member of the family Flavobacteriaceae isolated from tidal flat sediment. Antonie van Leeuwenhoek 104:225–231 [View Article][PubMed]
    [Google Scholar]
  25. Park S., Park D.-S., Bae K. S., Yoon J.-H. 2014; Phaeobacter aquaemixtae sp. nov., isolated from the junction between the ocean and a freshwater spring. Int J Syst Evol Microbiol 64:1378–1383 [View Article][PubMed]
    [Google Scholar]
  26. Parte A. C. 2014; LPSN–list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42:(D1)D613–D616 [View Article][PubMed]
    [Google Scholar]
  27. Piñeiro-Vidal M., Riaza A., Santos Y. 2008; Tenacibaculum discolor sp. nov. and Tenacibaculum gallaicum sp. nov., isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems. Int J Syst Evol Microbiol 58:21–25 [View Article][PubMed]
    [Google Scholar]
  28. Piñeiro-Vidal M., Gijón D., Zarza C., Santos Y. 2012; Tenacibaculum dicentrarchi sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from European sea bass. Int J Syst Evol Microbiol 62:425–429 [View Article][PubMed]
    [Google Scholar]
  29. Reichenbach H. 1992; The order Cytophagales. In The Prokaryotes, 2nd edn. vol. 4 pp 3631–3675Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. New York: Springer; [CrossRef]
    [Google Scholar]
  30. Sasser M. 1990 Identification of bacteria by gas chromatography of cellular fatty acids MIDI Technical Note 101 Newark, DE: MIDI Inc;
    [Google Scholar]
  31. Sheu S.-Y., Lin K.-Y., Chou J.-H., Chang P.-S., Arun A. B., Young C.-C., Chen W.-M. 2007; Tenacibaculum litopenaei sp. nov., isolated from a shrimp mariculture pond. Int J Syst Evol Microbiol 57:1148–1153 [View Article][PubMed]
    [Google Scholar]
  32. Stackebrandt E., Goebel B. M. 1994; Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44:846–849 [View Article]
    [Google Scholar]
  33. Staley J. T. 1968; Prosthecomicrobium and Ancalomicrobium: new prosthecate freshwater bacteria. J Bacteriol 95:1921–1942[PubMed]
    [Google Scholar]
  34. Suzuki M., Nakagawa Y., Harayama S., Yamamoto S. 2001; Phylogenetic analysis and taxonomic study of marine Cytophaga-like bacteria: proposal for Tenacibaculum gen. nov. with Tenacibaculum maritimum comb. nov. and Tenacibaculum ovolyticum comb. nov., and description of Tenacibaculum mesophilum sp. nov. and Tenacibaculum amylolyticum sp. nov. Int J Syst Evol Microbiol 51:1639–1652 [View Article][PubMed]
    [Google Scholar]
  35. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  36. Wang J.-T., Chou Y.-J., Chou J.-H., Chen C. A., Chen W.-M. 2008; Tenacibaculum aiptasiae sp. nov., isolated from a sea anemone Aiptasia pulchella . Int J Syst Evol Microbiol 58:761–766 [View Article][PubMed]
    [Google Scholar]
  37. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  38. Yoon J.-H., Kim H., Kim S.-B., Kim H.-J., Kim W. Y., Lee S. T., Goodfellow M., Park Y.-H. 1996; Identification of Saccharomonospora strains by the use of genomic DNA fragments and rRNA gene probes. Int J Syst Bacteriol 46:502–505 [View Article]
    [Google Scholar]
  39. Yoon J.-H., Lee S. T., Park Y.-H. 1998; Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48:187–194 [View Article][PubMed]
    [Google Scholar]
  40. Yoon J.-H., Kim I.-G., Shin D.-Y., Kang K. H., Park Y.-H. 2003; Microbulbifer salipaludis sp. nov., a moderate halophile isolated from a Korean salt marsh. Int J Syst Evol Microbiol 53:53–57 [View Article][PubMed]
    [Google Scholar]
  41. Yoon J.-H., Kang S.-J., Oh T.-K. 2005; Tenacibaculum lutimaris sp. nov., isolated from a tidal flat in the Yellow Sea, Korea. Int J Syst Evol Microbiol 55:793–798 [View Article][PubMed]
    [Google Scholar]
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