1887

Abstract

The taxonomic status of the bacterium is described, and based on the evidence presented, transfer of this species to the genus as comb. nov. is proposed. This reclassification is supported by data generated from genomic comparisons of ATCC VR-331 ( = FSC845 = DSM 101678) to other near neighbours, including subsp. . The full-length 16S rRNA gene sequence of strain ATCC VR-331 had 98.5 % nucleotide identity to the cognate gene in , with the highest similarity to subspecies . Phylogenetic trees of full-length 16S rRNA gene, and sequences from species of the genera (class ) and (class ) indicated that ATCC VR-331 was most closely related to members of the genus and not . Local collinear blocks within the chromosome of strain ATCC VR-331 had considerable similarity with subsp. , but not with any strain. The genomes of strain ATCC VR-331 and subsp. Utah 112 ( = ATCC 15482) contained an average nucleotide identity mean of 88.72 % and median of 89.18 %. Importantly, the genome of strain ATCC VR-331 contained one Pathogenicity Island, similar to subsp. , as well as the -specific gene and -specific genes and (also referred to as ). In contrast to the obligate intracellular genus , strain ATCC VR-331 and facultative intracellular can replicate in specialized cell-free media. Collectively, these results demonstrate that should be reclassified in the genus as comb. nov. The type strain of comb. nov. is ATCC VR-331 ( = FSC845 = DSM 101678). An emended description of the family is also provided.

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2016-03-01
2024-03-28
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