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Abstract

A bacterial strain, designated shin9-1, was isolated from a water sample taken from a water convolvulus field in Taiwan and characterized using a polyphasic taxonomical approach. Cells of strain shin9-1 were aerobic, Gram-stain-negative, rod-shaped and surrounded by a thick capsule and formed cream-coloured colonies. Growth occurred at 10–45 °C (optimum, 30 °C), with 0–3.0 % NaCl (optimum, 0.5 %) and at pH 7.0–9.0 (optimum, pH 7.0). Strain shin9-1 did not form nodules on a legume plant, , and the nodulation genes , and the nitrogenase reductase gene were not detected by PCR. Phylogenetic analyses based on 16S rRNA and three housekeeping gene sequences (, and ) showed that strain shin9-1 belonged to the genus . Strain shin9-1 had the highest level of 16S rRNA gene sequence similarity with respect to L61 (97.6 %). The major fatty acid of strain shin9-1 was Cω7. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, phosphatidylmonomethylethanolamine and several uncharacterized lipids. The DNA G+C content was 58.3 mol%. The DNA–DNA relatedness of strain shin9-1 with respect to recognized species of the genus was less than 70 %. Phenotypic characteristics of the novel strain also differed from those of the most closely related species of the genus . On the basis of the phylogenetic inference and phenotypic data, strain shin9-1 should be classified as a representative of a novel species, for which the name sp. nov. is proposed. The type strain is shin9-1 ( = LMG 27163 = KCTC 32148).

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2016-04-01
2024-03-29
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