RT Journal Article SR Electronic(1) A1 Li, Yan-Qiong A1 Li, Li A1 Fu, Yu-Song A1 Cui, Zhao-Qiong A1 Duan, Yan-Qing A1 Salam, Nimaichand A1 Guo, Jian-Wei A1 Chen, Wei A1 Li, Wen-JunYR 2016 T1 Pseudoclavibacter endophyticus sp. nov., isolated from roots of Glycyrrhiza uralensis JF International Journal of Systematic and Evolutionary Microbiology, VO 66 IS 3 SP 1287 OP 1292 DO https://doi.org/10.1099/ijsem.0.000876 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-positive, aerobic, rod-shaped, non-motile actinomycete strain, designated EGI 60007T, was isolated from healthy roots of Glycyrrhiza uralensis F. collected from Yili County, Xinjiang Province, north-west China. A polyphasic approach was applied to study the taxonomic position of the new isolate. The 16S rRNA gene sequence of strain EGI 60007T had highest similarities with members of the genus Pseudoclavibacter, including Pseudoclavibacter chungangensis CAU 59T (96.98 % 16S rRNA gene sequence similarity), Pseudoclavibacter helvolus DSM 20419T (96.43 %) and Pseudoclavibacter terrae THG-MD12T (96.14 %). The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60007T clustered with members of the genus Pseudoclavibacter, and formed a distinct clade with P. chungangensis CAU 59T. The polar lipids detected for strain EGI 60007T were phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified lipid. The DNA G+C content was determined to be 63.3 mol%. The chemotaxonomic features of strain EGI 60007T showed typical characteristics of the genus Pseudoclavibacter, with MK-9 as the respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan, and anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0 as the major fatty acids. The sugars of whole-cell hydrolysates were mainly mannose, rhamnose, ribose and glucose, and a minor amount of xylose. Based on the results of the phylogentic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60007T is considered to represent a novel species of the genus Pseudoclavibacter, for which the name Pseudoclavibacter endophyticus sp. nov. is proposed. The type strain is EGI 60007T ( = CGMCC 1.15081T = KCTC 39112T = DSM 29943T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000876