@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000894, author = "Kern, Tobias and Fischer, Martin A. and Deppenmeier, Uwe and Schmitz, Ruth A. and Rother, Michael", title = "Methanosarcina flavescens sp. nov., a methanogenic archaeon isolated from a full-scale anaerobic digester", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "3", pages = "1533-1538", doi = "https://doi.org/10.1099/ijsem.0.000894", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000894", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel, strictly anaerobic, methanogenic archaeon, strain E03.2T, was isolated from a full-scale biogas plant in Germany. Cells were non-motile sarcina-like cocci, occurring in aggregates. Strain E03.2T grew autotrophically on H2 plus CO2, and additionally cells could utilize acetate, methanol, moni-, di- and trimethylamine as carbon and energy sources; however, growth or methanogenesis on formate was not observed. Yeast extract and vitamins stimulated growth but were not mandatory. The optimal growth temperature of strain E03.2T was approximately 45 °C; maximal growth rates were obtained at about pH 7.0 in the presence of approximately 6.8 mM NaCl. The DNA G+C content of strain E03.2T was 41.3 mol%. Phylogenetic analyses based on 16S rRNA gene and mcrA sequences placed strain E03.2T within the genus Methanosarcina. Based on 16S rRNA gene sequence similarity strain E03.2T was related to seven different species of the genus Methanosarcina, but most closely related to Methanosarcina thermophila TM-1T. Phenotypic, physiological and genomic characteristics indicated that strain E03.2T represents a novel species of the genus Methanosarcina, for which the name Methanosarcina flavescens sp. nov. is proposed. The type strain is E03.2T ( = DSM 100822T = JCM 30921T).", }