@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000898, author = "Bird, Philip S. and Trott, Darren J. and Mikkelsen, Deirdre and Milinovich, Gabriel J. and Hillman, Kristine M. and Burrell, Paul C. and Blackall, Linda L.", title = "Porphyromonas loveana sp. nov., isolated from the oral cavity of Australian marsupials", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "10", pages = "3771-3778", doi = "https://doi.org/10.1099/ijsem.0.000898", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000898", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "16S rRNA gene sequencing", keywords = "MLST, glutamate dehydrogenase gene sequencing", keywords = "MALDI-TOF", keywords = "Porphyromonas", keywords = "16S-23S rRNA internal transcribed spacer region sequencing", keywords = "marsupial", abstract = "An obligatory anaerobic, Gram-stain-negative coccobacillus with black-pigmented colonies was isolated from the oral cavity of selected Australian marsupial species. Phenotypic and molecular criteria showed that this bacterium was a distinct species within the genus Porphyromonas , and was closely related to Porphyromonas gingivalis and Porphyromonas gulae . This putative novel species and P. gulae could be differentiated from P. gingivalis by catalase activity. Further characterization by multi-locus enzyme electrophoresis of glutamate dehydrogenase and malate dehydrogenase enzyme mobility and matrix-assisted laser desorption ionization time-of-flight MS showed that this putative novel species could be differentiated phenotypically from P. gingivalis and P. gulae . Definitive identification by 16S rRNA gene sequencing showed that this bacterium belonged to a unique monophyletic lineage, phylogenetically distinct from P. gingivalis (94.9ā€‰% similarity) and P. gulae (95.5ā€‰%). This also was supported by 16Sā€“23S rRNA intergenic spacer region and glutamate dehydrogenase gene sequencing. A new species epithet, Porphyromonas loveana sp. nov., is proposed for this bacterium, with DSM 28520T (=NCTC 13658T=UQD444T=MRK101T), isolated from a musky rat kangaroo, as the type strain.", }