1887

Abstract

A moderately halophilic bacterium was isolated from a brine sample of a hypersaline lake, Aran-Bidgol, in Iran. The strain, designated J8B, was Gram-stain-positive, endospore-forming, rod-shaped, strictly aerobic, motile and produced cream colonies. Strain J8B grew in NaCl at between 3.0–15.0 % (w/v) (optimally at 7.5 % NaCl, w/v), between pH 6.5–9.0 (optimally at pH 8.0) and between 20–45 °C (optimally at 35 °C). Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain J8B is a member of the genus and most closely related to CL-MP28, SA9 and R-2 (96.9 %, 96.3 % and 96.2 % similarities, respectively). The level of DNA–DNA relatedness between the novel isolate and IBRC-M 10567 was 10 %. The major cellular fatty acids of the isolate were anteiso-C, iso-C and anteiso-C. The polar lipid pattern of strain J8B consisted of phosphatidylglycerol, diphosphatidylglycerol, five phospholipids, two aminolipids and two glycoaminolipids. It contained MK-7 as the predominant menaquinone and -diaminopimelic acid in the cell-wall peptidoglycan. The G+C content of the genomic DNA of this strain was 39.2 mol%. Phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data suggest that this strain represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is strain J8B ( = IBRC-M 10444 = DSM 23996).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000952
2016-03-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/3/1317.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000952&mimeType=html&fmt=ahah

References

  1. Amoozegar M. A., Bagheri M., Makhdoumi-Kakhki A., Didari M., Schumann P., Spröer C., Sánchez-Porro C., Ventosa A. 2014; Oceanobacillus limi sp. nov., a moderately halophilic bacterium from a salt lake. Int J Syst Evol Microbiol 64:1284–1289 [View Article][PubMed]
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. 1977; A rapid method for the base ratio determination of bacterial DNA. Anal Biochem 81:461–466 [View Article][PubMed]
    [Google Scholar]
  3. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  4. Euzéby J. P. 1997; List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47:590–592 [View Article][PubMed]
    [Google Scholar]
  5. Felsenstein J. 1981; Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17:368–376 [View Article][PubMed]
    [Google Scholar]
  6. Fitch W. M. 1971; Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20:406–416 [View Article]
    [Google Scholar]
  7. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [View Article][PubMed]
    [Google Scholar]
  8. Harrigan W. F., McCance M. E. 1976 Laboratory Methods in Food and Dairy Microbiology London: Academic Press;
    [Google Scholar]
  9. Hirota K., Hanaoka Y., Nodasaka Y., Yumoto I. 2013; Oceanobacillus polygoni sp. nov., a facultatively alkaliphile isolated from indigo fermentation fluid. Int J Syst Evol Microbiol 63:3307–3312 [View Article][PubMed]
    [Google Scholar]
  10. Huss V. A., Festl H., Schleifer K. H. 1983; Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4:184–192 [View Article][PubMed]
    [Google Scholar]
  11. Kämpfer P., Kroppenstedt R. M. 1996; Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42:989–1005 [View Article]
    [Google Scholar]
  12. Kim Y. G., Choi D. H., Hyun S., Cho B. C. 2007; Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. Int J Syst Evol Microbiol 57:409–413 [View Article][PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  14. Kim W., Siamphan C., Kim J.-H., Sukhoom A. 2015; Oceanobacillus arenosus sp. nov., a moderate halophilic bacterium isolated from marine sand. Int J Syst Evol Microbiol. 65:2943–2948 [View Article]
    [Google Scholar]
  15. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. 1985; Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A 82:6955–6959 [View Article][PubMed]
    [Google Scholar]
  16. Lee J.-S., Lim J.-M., Lee K. C., Lee J.-C., Park Y.-H., Kim C.-J. 2006; Virgibacillus koreensis sp. nov., a novel bacterium from a salt field, and transfer of Virgibacillus picturae to the genus Oceanobacillus as Oceanobacillus picturae comb. nov. with emended descriptions. Int J Syst Evol Microbiol 56:251–257 [View Article][PubMed]
    [Google Scholar]
  17. Lee S. Y., Oh T. K., Kim W., Yoon J. H. 2010; Oceanobacillus locisalsi sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 60:2758–2762 [View Article][PubMed]
    [Google Scholar]
  18. Lee D. C., Kang H., Weerawongwiwat V., Kim B., Choi Y.-W., Kim W. 2013; Oceanobacillus chungangensis sp. nov., isolated from a sand dune. Int J Syst Evol Microbiol 63:3666–3671 [View Article][PubMed]
    [Google Scholar]
  19. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L., other authors. 2009; Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. Int J Syst Evol Microbiol 59:2114–2121 [View Article][PubMed]
    [Google Scholar]
  20. Lu J., Nogi Y., Takami H. 2001; Oceanobacillus iheyensis gen. nov., sp. nov., a deep-sea extremely halotolerant and alkaliphilic species isolated from a depth of 1050 m on the Iheya Ridge. FEMS Microbiol Lett 205:291–297 [View Article][PubMed]
    [Google Scholar]
  21. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S., other authors. 2004; arb: a software environment for sequence data. Nucleic Acids Res 32:1363–1371 [View Article][PubMed]
    [Google Scholar]
  22. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. 2002; A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25:360–375 [View Article][PubMed]
    [Google Scholar]
  23. Mayr R., Busse H. J., Worliczek H. L., Ehling-Schulz M., Scherer S. 2006; Ornithinibacillus gen. nov., with the species Ornithinibacillus bavariensis sp. nov. and Ornithinibacillus californiensis sp. nov. Int J Syst Evol Microbiol 56:1383–1389 [View Article][PubMed]
    [Google Scholar]
  24. Mesbah M., Premachandran U., Whitman W. B. 1989; Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Evol Bacteriol 39:159–167 [View Article]
    [Google Scholar]
  25. Murray R. G. E., Doetsch R. N., Robinow C. F. 1994; Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology pp 21–41Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  26. Nam J. H., Bae W., Lee D. H. 2008; Oceanobacillus caeni sp. nov., isolated from a Bacillus-dominated wastewater treatment system in Korea. Int J Syst Evol Microbiol 58:1109–1113 [View Article][PubMed]
    [Google Scholar]
  27. Namwong S., Tanasupawat S., Lee K. C., Lee J.-S. 2009; Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand. Int J Syst Evol Microbiol 59:2254–2259 [View Article][PubMed]
    [Google Scholar]
  28. Parte A. C. 2014; LPSN—list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42:(D1)D613–D616 [View Article][PubMed]
    [Google Scholar]
  29. Quesada E., Ventosa A., Ruiz-Berraquero F., Ramos-Cormenzana A. 1984; Deleya halophila, a new species of moderately halophilic bacteria. Int J Syst Bacteriol 34:287–292 [View Article]
    [Google Scholar]
  30. Rhuland L. E., Work E., Denman R. F., Hoare D. S. 1955; The behavior of the isomers of α,ϵ-diaminopimelic acid on paper chromatograms. J Am Chem Soc 77:4844–4846 [View Article]
    [Google Scholar]
  31. Saitou N., Nei M. 1987; The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425[PubMed]
    [Google Scholar]
  32. Smibert R. M., Krieg N. R. 1994; Phenotypic characterization. In Methods for General and Molecular Bacteriology pp 607–654Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  33. Tindall B. J., Rosselló-Móra R., Busse H.-J., Ludwig W., Kämpfer P. 2010; Notes on the characterization of prokaryote strains for taxonomic purposes. Int J Syst Evol Microbiol 60:249–266 [View Article][PubMed]
    [Google Scholar]
  34. Tominaga T., An S. Y., Oyaizu H., Yokota A. 2009; Oceanobacillus soja sp. nov. isolated from soy sauce production equipment in Japan. J Gen Appl Microbiol 55:225–232 [View Article][PubMed]
    [Google Scholar]
  35. Ventosa A., Quesada E., Rodríguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; Numerical taxonomy of moderately halophilic Gram-negative rods. Microbiol 128:1959–1968 [CrossRef]
    [Google Scholar]
  36. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  37. Whon T. W., Jung M. J., Roh S. W., Nam Y. D., Park E. J., Shin K. S., Bae J. W. 2010; Oceanobacillus kimchii sp. nov. isolated from a traditional Korean fermented food. J Microbiol 48:862–866 [View Article][PubMed]
    [Google Scholar]
  38. Wu M., Yang G., Yu Z., Zhuang L., Jin Y., Zhou S. 2014; Oceanobacillus luteolus sp. nov., isolated from soil. Int J Syst Evol Microbiol 64:1495–1500 [View Article][PubMed]
    [Google Scholar]
  39. Yang J. Y., Huo Y. Y., Xu X. W., Meng F. X., Wu M., Wang C. S. 2010; Oceanobacillus neutriphilus sp. nov., isolated from activated sludge in a bioreactor. Int J Syst Evol Microbiol 60:2409–2414 [View Article][PubMed]
    [Google Scholar]
  40. Yu C., Yu S., Zhang Z., Li Z., Zhang X. H. 2014; Oceanobacillus pacificus sp. nov., isolated from a deep-sea sediment. Int J Syst Evol Microbiol 64:1278–1283 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000952
Loading
/content/journal/ijsem/10.1099/ijsem.0.000952
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error