1887

Abstract

A Gram-stain-positive bacterium, designated T14, was isolated from the gut of larvae and was subjected to a taxonomic study. The isolate was rod-shaped, aerobic, non-motile, non-spore-forming and yellow-pigmented. Phylogenetic analysis based on 16S rRNA gene sequence comparison indicated that the isolate is related to the genus . Its closest neighbours were the type strains ‘’ F3-P9 (96.8 % 16S rRNA gene sequence similarity), NAL101 (96.2 %) and DSM 19883 (95.5 %). The DNA G+C content of strain T14 was 69.3 mol%, and DNA–DNA hybridization values with closely related strains were < 32 %. The predominant cellular fatty acids were anteiso-C (49.3 %), iso-C (16.4 %) and anteiso-C (16.8 %). The major polar lipids were aminolipid, diphosphatidylglycerol, phosphatidylglycerol, phospholipid, phosphoglycolipid and unidentified glycolipids. The predominant respiratory quinone was MK-11. Based on these phylogenetic and phenotypic results, strain T14 can be clearly distinguished from all of the recognized species of the genus and is considered to represent a novel species of the genus The name sp. nov. is proposed, with the type strain T14 ( = DSM 28968 = JCM 30245).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.000957
2016-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/66/4/1857.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.000957&mimeType=html&fmt=ahah

References

  1. Collins M. D., Jones D. 1981; Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45:316–354[PubMed]
    [Google Scholar]
  2. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12:133–142 [View Article][PubMed]
    [Google Scholar]
  3. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. 1996; Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:234–239 [View Article][PubMed]
    [Google Scholar]
  4. Halpern M., Shakéd T., Pukall R., Schumann P. 2009; Leucobacter chironomi sp. nov., a chromate-resistant bacterium isolated from a chironomid egg mass. Int J Syst Evol Microbiol 59:665–670 [View Article][PubMed]
    [Google Scholar]
  5. Hungate R. E. 1969; A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B:117–132 [CrossRef]
    [Google Scholar]
  6. Kim H. J., Lee S. S. 2011; Leucobacter kyeonggiensis sp. nov., a new species isolated from dye waste water. J Microbiol 49:1044–1049 [View Article][PubMed]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H., other authors. 2012; Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 62:716–721 [View Article][PubMed]
    [Google Scholar]
  8. Kumar S., Tamura K., Nei M. 2004; mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5:150–163 [View Article][PubMed]
    [Google Scholar]
  9. Lee J. H., Lee S. S. 2012; Leucobacter margaritiformis sp. nov., isolated from bamboo extract. Curr Microbiol 64:441–448 [View Article][PubMed]
    [Google Scholar]
  10. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5:109–118 [View Article][PubMed]
    [Google Scholar]
  11. Morais P. V., Paulo C., Francisco R., Branco R., Paula Chung A., da Costa M. S. 2006; Leucobacter luti sp. nov., and Leucobacter alluvii sp. nov., two new species of the genus Leucobacter isolated under chromium stress. Syst Appl Microbiol 29:414–421 [View Article][PubMed]
    [Google Scholar]
  12. Owen R. J., Pitcher D. 1985; Current methods for estimating DNA base composition and levels of DNA-DNA hybridization. In Chemical Methods in Bacterial Systematics pp 67–93Edited by Goodfellow M., Minnikin D. E. London: Academic Press;
    [Google Scholar]
  13. Reddy C. A., Beveridge T. J., Breznak J. A., Marzluf G. A., Schmidt T. M., Snyder L. R. editors 2007 Methods for general and molecular microbiology, 3rd edn. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  14. Schleifer K. H. 1985; Analysis of the chemical composition and primary structure of murein. Methods Microbiol 18:123–156 [View Article]
    [Google Scholar]
  15. Schleifer K. H., Kandler O. 1972; Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36:407–477[PubMed]
    [Google Scholar]
  16. Shin N. R., Kim M. S., Jung M. J., Roh S. W., Nam Y. D., Park E. J., Bae J. W. 2011; Leucobacter celer sp. nov., isolated from Korean fermented seafood. Int J Syst Evol Microbiol 61:2353–2357 [View Article][PubMed]
    [Google Scholar]
  17. Sturm G., Jacobs J., Spröer C., Schumann P., Gescher J. 2011; Leucobacter chromiiresistens sp. nov., a chromate-resistant strain. Int J Syst Evol Microbiol 61:956–960 [View Article][PubMed]
    [Google Scholar]
  18. Takeuchi M., Weiss N., Schumann P., Yokota A. 1996; Leucobacter komagatae gen. nov., sp. nov., a new aerobic Gram-positive, nonsporulating rod with 2,4-diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46:967–971 [View Article][PubMed]
    [Google Scholar]
  19. Thompson J. D., Higgins D. G., Gibson T. J. 1994; clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680 [View Article][PubMed]
    [Google Scholar]
  20. Tindall B. J. 1990; A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 13:128–130 [View Article]
    [Google Scholar]
  21. Ue H. 2011; Leucobacter exalbidus sp. nov., an actinobacterium isolated from a mixed culture from compost. J Gen Appl Microbiol 57:27–33 [View Article][PubMed]
    [Google Scholar]
  22. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E., other authors. 1987; International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37:463–464 [View Article]
    [Google Scholar]
  23. Weon H. Y., Anandham R., Tamura T., Hamada M., Kim S. J., Kim Y. S., Suzuki K., Kwon S. W. 2012; Leucobacter denitrificans sp. nov., isolated from cow dung. J Microbiol 50:161–165 [View Article][PubMed]
    [Google Scholar]
  24. Zhu D., Tanabe S. H., Xie C., Honda D., Sun J., Ai L. 2014; Bacillus ligniniphilus sp. nov., an alkaliphilic and halotolerant bacterium isolated from sediments of the South China Sea. Int J Syst Evol Microbiol 64:1712–1717 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.000957
Loading
/content/journal/ijsem/10.1099/ijsem.0.000957
Loading

Data & Media loading...

Supplements

Supplementary Data

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error