@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.000976, author = "Wu, Hao and Liu, Bin and Pan, Shangli", title = "Saccharopolyspora subtropica sp. nov., a thermophilic actinomycete isolated from soil of a sugar cane field", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2016", volume = "66", number = "5", pages = "1990-1995", doi = "https://doi.org/10.1099/ijsem.0.000976", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.000976", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "A novel thermophilic actinomycete, designated strain T3T, was isolated from a soil sample of a sugar cane field. The strain grew at 25–60 °C (optimum 37–50 °C), at pH 6.0–11.0 (optimum 7.0–9.0) and with 0–12.0 % (w/v) NaCl (optimum 0–7 %). The aerial mycelium was white and the vegetative mycelium was colourless to pale yellow. The substrate mycelium fragmented into rod-shaped elements after 4–5 days at 50 °C. The aerial mycelium formed flexuous chains of 5–20 spores per chain; the oval-shaped spores had spiny surfaces and were non-motile. The organism contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars consisted of arabinose, galactose and ribose. The cellular fatty acid profile consisted mainly of anteiso-C17 : 0, iso-C17 : 0 and iso-C16 : 0. The quinone system was composed predominantly of MK-9(H4). The phospholipids detected were diphosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, phosphatidylethanolamine, phosphatidylmethylethanolamine and ninhydrin-positive glycophospholipids. The DNA G+C content of strain T3T was 71.3 mol%. The organism showed a combination of morphological and chemotaxonomic properties typical of members of the genus Saccharopolyspora. In the 16S rRNA gene tree of Saccharopolyspora it formed a distinct phyletic line and was related most closely to Saccharopolyspora thermophila 216T. However, the phenotypic characteristics of strain T3T were significantly different from those of S. thermophila 216T and DNA–DNA hybridization revealed a low level of relatedness (28.6–32.3 %) between them. Based on the phenotypic and phylogenetic data, strain T3T represents a novel species in the genus Saccharopolyspora, for which the name Saccharopolyspora subtropica sp. nov. is proposed. The type strain is T3T ( = DSM 46801T = CGMCC 4.7206T).", }