1887

Abstract

An endohyphal bacterium (strain B1-EB) living in association with the fungus FMR23-6 I-B1 was isolated from a fungal cell homogenate and studied for its taxonomic allocation. Cells were Gram-stain-negative, rod-shaped, non-spore-forming, non-motile, and negative for oxidase and catalase. Strain B1-EB required cysteine for growth and grew at temperatures between 4 and 35 °C. A comparative analysis of 16S rRNA gene sequences revealed that strain B1-EB forms a distinct clade in the family , encompassing a group of endosymbionts associated with several soil isolates of . The most closely related genus is ‘ Glomeribacter gigasporarum’, an endosymbiont of the arbuscular mycorrhizal fungus . The major cellular fatty acids of strain B1-EB were C, summed feature 3 (Cω7 and Cω6) and summed feature 8 (Cω7 or Cω6). Ubiquinone Q-8 was the only quinone detected. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, an unknown aminophospholipid and two unknown aminolipids. The DNA G+C content was 49.8 mol%. On the basis of phenotypic, chemotaxonomic, and phylogenetic characteristics, strain B1-EB represents a novel genus and novel species in the family , for which the name gen. nov., sp. nov. is proposed. The type strain is B1-EB ( = JCM 30646 = LMG 28693 = NBRC 110909).

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2016-05-01
2024-03-29
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References

  1. Amann R. I., Binder B. J., Olson R. J., Chisholm S. W., Devereux R., Stahl D. A. 1990; Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl Environ Microbiol 56:1919–1925[PubMed]
    [Google Scholar]
  2. Anandham R., Indiragandhi P., Kwon S. W., Sa T. M., Jeon C. O., Kim Y. K., Jee H. J. 2010; Pandoraea thiooxydans sp. nov., a facultatively chemolithotrophic, thiosulfate-oxidizing bacterium isolated from rhizosphere soils of sesame (Sesamum indicum L.). Int J Syst Evol Microbiol 60:21–26 [View Article][PubMed]
    [Google Scholar]
  3. Barbieri E., Potenza L., Rossi I., Sisti D., Giomaro G., Rossetti S., Beimfohr C., Stocchi V. 2000; Phylogenetic characterization and in situ detection of a Cytophaga-Flexibacter-Bacteroides phylogroup bacterium in Tuber borchii vittad. ectomycorrhizal mycelium. Appl Environ Microbiol 66:5035–5042 [View Article][PubMed]
    [Google Scholar]
  4. Bianciotto V., Lumini E., Bonfante P., Vandamme P. 2003; Candidatus glomeribacter gigasporarum’ gen. nov., sp. nov., an endosymbiont of arbuscular mycorrhizal fungi. Int J Syst Evol Microbiol 53:121–124 [View Article][PubMed]
    [Google Scholar]
  5. Chang S.-C., Wang J.-T., Vandamme P., Hwang J.-H., Chang P.-S., Chen W.-M. 2004; Chitinimonas taiwanensis gen. nov., sp. nov., a novel chitinolytic bacterium isolated from a freshwater pond for shrimp culture. Syst Appl Microbiol 27:43–49 [View Article][PubMed]
    [Google Scholar]
  6. Coenye T., Falsen E., Hoste B., Ohlén M., Goris J., Govan J. R., Gillis M., Vandamme P. 2000; Description of Pandoraea gen. nov. with Pandoraea apista sp. nov., Pandoraea pulmonicola sp. nov., Pandoraea pnomenusa sp. nov., Pandoraea sputorum sp. nov. and Pandoraea norimbergensis comb. nov. Int J Syst Evol Microbiol 50:887–899 [View Article][PubMed]
    [Google Scholar]
  7. Coenye T., Goris J., De Vos P., Vandamme P., LiPuma J. J. 2003; Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov. Int J Syst Evol Microbiol 53:1075–1080 [View Article][PubMed]
    [Google Scholar]
  8. Daneshvar M. I., Douglas M. P., Weyant R. S. 2001; Cellular fatty acid composition of Lautropia mirabilis . J Clin Microbiol 39:4160–4162 [View Article][PubMed]
    [Google Scholar]
  9. Desirò A., Salvioli A., Ngonkeu E. L., Mondo S. J., Epis S., Faccio A., Kaech A., Pawlowska T. E., Bonfante P. 2014; Detection of a novel intracellular microbiome hosted in arbuscular mycorrhizal fungi. ISME J 8:257–270 [View Article][PubMed]
    [Google Scholar]
  10. Edgar R. C. 2004; muscle: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32:1792–1797 [View Article][PubMed]
    [Google Scholar]
  11. Feeley J. C., Gibson R. J., Gorman G. W., Langford N. C., Rasheed J. K., Mackel D. C., Baine W. B. 1979; Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila . J Clin Microbiol 10:437–441[PubMed]
    [Google Scholar]
  12. Fujimura R., Nishimura A., Ohshima S., Sato Y., Nishizawa T., Oshima K., Hattori M., Narisawa K., Ohta H. 2014; Draft genome sequence of the betaproteobacterial endosymbiont associated with the fungus Mortierella elongata FMR23-6. Genome Announc 2:e01272–14-e01214 [View Article][PubMed]
    [Google Scholar]
  13. Gerner-Smidt P., Keiser-Nielsen H., Dorsch M., Stackebrandt E., Ursing J., Blom J., Christensen A. C., Christensen J. J., Frederiksen W., other authors. 1994; Lautropia mirabilis gen. nov., sp. nov., a Gram-negative motile coccus with unusual morphology isolated from the human mouth. Microbiology 140:1787–1797 [View Article][PubMed]
    [Google Scholar]
  14. Gillis M., Van Van T., Bardin R., Goor M., Hebbar P., Willems A., Segers P., Kersters K., Heulin T., Fernandez M. P. 1995; Polyphasic taxonomy in the genus Burkholderia leading to an emended description of the genus and proposition of Burkholderia vietnamiensis sp. nov. for N2-fixing isolates from rice in Vietnam. Int J Syst Bacteriol 45:274–289 [CrossRef]
    [Google Scholar]
  15. Hahn M. W., Lang E., Brandt U., Wu Q. L., Scheuerl T. 2009; Emended description of the genus Polynucleobacter and the species Polynucleobacter necessarius and proposal of two subspecies, P. necessarius subsp. necessarius subsp. nov. and P. necessarius subsp. asymbioticus subsp. nov. Int J Syst Evol Microbiol 59:2002–2009 [View Article][PubMed]
    [Google Scholar]
  16. Hahn M. W., Minasyan A., Lang E., Koll U., Spröer C. 2012; Polynucleobacter difficilis sp. nov., a planktonic freshwater bacterium affiliated with subcluster B1 of the genus Polynucleobacter . Int J Syst Evol Microbiol 62:376–383 [View Article][PubMed]
    [Google Scholar]
  17. Hoffman M. T., Arnold A. E. 2010; Diverse bacteria inhabit living hyphae of phylogenetically diverse fungal endophytes. Appl Environ Microbiol 76:4063–4075 [View Article][PubMed]
    [Google Scholar]
  18. Huelsenbeck J. P., Ronquist F. 2001; mrbayes: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755 [View Article][PubMed]
    [Google Scholar]
  19. Ibrahim A. S., Gebremariam T., Liu M., Chamilos G., Kontoyiannis D., Mink R., Kwon-Chung K. J., Fu Y., Skory C. D., other authors. 2008; Bacterial endosymbiosis is widely present among zygomycetes but does not contribute to the pathogenesis of mucormycosis. J Infect Dis 198:1083–1090 [View Article][PubMed]
    [Google Scholar]
  20. Jendrossek D. 2001; Transfer of [Pseudomonas] lemoignei, a Gram-negative rod with restricted catabolic capacity, to Paucimonas gen. nov. with one species, Paucimonas lemoignei comb. nov. Int J Syst Evol Microbiol 51:905–908 [View Article][PubMed]
    [Google Scholar]
  21. Joung Y., Lee B.-I., Kang H., Kim H., Joh K. 2014; Chitinimonas viridis sp. nov., isolated from a mesotrophic artificial lake. Int J Syst Evol Microbiol 64:1123–1126 [View Article][PubMed]
    [Google Scholar]
  22. Kai K., Furuyabu K., Tani A., Hayashi H. 2012; Production of the quorum-sensing molecules N-acylhomoserine lactones by endobacteria associated with Mortierella alpina A-178. ChemBioChem 13:1776–1784 [View Article][PubMed]
    [Google Scholar]
  23. Kim B.-Y., Weon H.-Y., Yoo S.-H., Chen W.-M., Kwon S.-W., Go S.-J., Stackebrandt E. 2006; Chitinimonas koreensis sp. nov., isolated from greenhouse soil in Korea. Int J Syst Evol Microbiol 56:1761–1764 [View Article][PubMed]
    [Google Scholar]
  24. Lackner G., Möbius N., Scherlach K., Partida-Martinez L. P., Winkler R., Schmitt I., Hertweck C. 2009; Global distribution and evolution of a toxinogenic Burkholderia-Rhizopus symbiosis. Appl Environ Microbiol 75:2982–2986 [View Article][PubMed]
    [Google Scholar]
  25. Li Y., Zhu H., Lai Q., Lei X., Chen Z., Zhang H., Tian Y., Zheng W., Zheng T. 2014; Chitinimonas prasina sp. nov., isolated from lake water. Int J Syst Evol Microbiol 64:3005–3009 [View Article][PubMed]
    [Google Scholar]
  26. Lu H., Sato Y., Fujimura R., Nishizawa T., Kamijo T., Ohta H. 2011; Limnobacter litoralis sp. nov., a thiosulfate-oxidizing, heterotrophic bacterium isolated from a volcanic deposit, and emended description of the genus Limnobacter . Int J Syst Evol Microbiol 61:404–407 [View Article][PubMed]
    [Google Scholar]
  27. Minnikin D. E., Collins M. D., Goodfellow M. 1979; Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47:87–95 [View Article]
    [Google Scholar]
  28. Mosse B. 1970; Honey-coloured, sessile Endogone spores. Arch Mikrobiol 74:146–159 [View Article]
    [Google Scholar]
  29. Naumann M., Schüssler A., Bonfante P. 2010; The obligate endobacteria of arbuscular mycorrhizal fungi are ancient heritable components related to the Mollicutes . ISME J 4:862–871 [View Article][PubMed]
    [Google Scholar]
  30. Ohta H., Hattori T. 1983; Agromonas oligotrophica gen. nov., sp. nov., a nitrogen-fixing oligotrophic bacterium. Antonie van Leeuwenhoek 49429–446[PubMed]
    [Google Scholar]
  31. Partida-Martinez L. P., Groth I., Schmitt I., Richter W., Roth M., Hertweck C. 2007; Burkholderia rhizoxinica sp. nov. and Burkholderia endofungorum sp. nov., bacterial endosymbionts of the plant-pathogenic fungus Rhizopus microsporus . Int J Syst Evol Microbiol 57:2583–2590 [View Article][PubMed]
    [Google Scholar]
  32. Quast C., Pruesse E., Yilmaz P., Gerken J., Schweer T., Yarza P., Peplies J., Glöckner F. O. 2013; The silva ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 41:(D1)D590–D596 [View Article][PubMed]
    [Google Scholar]
  33. Sahin N., Tani A., Kotan R., Sedlácek I., Kimbara K., Tamer A. U. 2011; Pandoraea oxalativorans sp. nov., Pandoraea faecigallinarum sp. nov. and Pandoraea vervacti sp. nov., isolated from oxalate-enriched culture. Int J Syst Evol Microbiol 61:2247–2253 [View Article][PubMed]
    [Google Scholar]
  34. Sato Y., Narisawa K., Tsuruta K., Umezu M., Nishizawa T., Tanaka K., Yamaguchi K., Komatsuzaki M., Ohta H. 2010; Detection of betaproteobacteria inside the mycelium of the fungus Mortierella elongata . Microbes Environ 25:321–324 [View Article][PubMed]
    [Google Scholar]
  35. Sharma M., Schmid M., Rothballer M., Hause G., Zuccaro A., Imani J., Kämpfer P., Domann E., Schäfer P., other authors. 2008; Detection and identification of bacteria intimately associated with fungi of the order Sebacinales. Cell Microbiol 10:2235–2246 [View Article][PubMed]
    [Google Scholar]
  36. Spring S., Kämpfer P., Schleifer K. H. 2001; Limnobacter thiooxidans gen. nov., sp. nov., a novel thiosulfate-oxidizing bacterium isolated from freshwater lake sediment. Int J Syst Evol Microbiol 51:1463–1470 [View Article][PubMed]
    [Google Scholar]
  37. Tamaoka J., Komagata K. 1984; Determination of DNA base composition by reverse phase high-performance liquid chromatography. FEMS Microbiol Lett 25:125–128 [View Article]
    [Google Scholar]
  38. Vandamme P., Coenye T. 2004; Taxonomy of the genus Cupriavidus: a tale of lost and found. Int J Syst Evol Microbiol 54:2285–2289 [View Article][PubMed]
    [Google Scholar]
  39. Vaneechoutte M., Kämpfer P., DeBaere T., Falsen E., Verschraegen G. 2004; Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov. Int J Syst Evol Microbiol 54:317–327 [View Article][PubMed]
    [Google Scholar]
  40. Yabuuchi E., Kosako Y., Oyaizu H., Yano I., Hotta H., Hashimoto Y., Ezaki T., Arakawa M. 1992; Proposal of Burkholderia gen. nov. and transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. nov. Microbiol Immunol 36:1251–1275 [View Article][PubMed]
    [Google Scholar]
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